基于斐林试剂检测柿霜中还原糖含量的实验设计  

Determination of Reducing Sugar Content in Persimmon Frost Based on Fehling’s Solution

作　　者：吕小兰[1] 彭邹君 芮嘉晗 徐楚楚 邱萍[1] LV Xiao-Lan;PENG Zou-Jun;RUI Jia-Han;XU Chu-Chu;QIU Ping(School of Chemistry and Chemical Engineering,Nanchang University,Nanchang 330031,China;Institute for Advanced Study,Nanchang University,Nanchang 330031,China)

机构地区：[1]南昌大学化学化工学院,江西南昌330031 [2]南昌大学高等研究院,江西南昌330031

出　　处：《化学教育（中英文）》2022年第20期62-66,共5页Chinese Journal of Chemical Education

基　　金：江西省高等学校教学改革研究课题(JXJG-20-1-48)。

摘　　要：柿饼表面的柿霜主要成分是还原糖,基于斐林试剂与还原糖发生氧化还原反应的原理,可利用稀释定律对柿霜中还原糖含量进行测定。在定量滴定分析中,对斐林试剂的组成、滴定方式以及滴定计算方法进行了探究,并探讨了反应时溶液pH、斐林试剂用量和温度对实验的影响。与经典的碘量法比较,本方法的精密度和准确度都比较理想,是一种更为简便的还原糖含量测定方法。本实验有助于增强学生的食品安全意识,提高学生在滴定分析中数据处理的能力,可替代碘量法成为分析化学实验中一个新的实验。In this paper, a new titration experiment was designed to detect reducing sugar content in persimmon frost based on the redox reaction between Fehling’s solution and reducing sugar. There comes lots of creation and progress in the Fehling’s solution composition, the titration method and the titration result calculation method. And the effects of pH, temperature and amount of Fehling’s solution on the color reaction were discussed in details. Finally, the results obtained by the proposed assay were compared with those by iodimetry with satisfactory results. This experiment helps to enhance students’ awareness of food safety and improve their ability to analyze multivariable problems. It can replace iodometry and become a new experiment in analytical chemistry experiment.

关 键 词：滴定分析 斐林试剂 还原糖 柿霜 颜色反应 实验设计 

分 类 号：TS255.7-4[轻工技术与工程—农产品加工及贮藏工程] G642.423[轻工技术与工程—食品科学与工程]