超甜玉米闽双色6号籽粒甜度遗传基础解析  

Genetic Basis of Sweetness of Super-sweet Corn,Minshuangse No.6

作　　者：林静[1] 张扬[1] 林建新[1] 卢和顶[1] 陈山虎[1] 廖长见[1] LIN Jing;ZHANG Yang;LIN Jianxin;LU Heding;CHEN Shanhu;LIAO Changjian(Institute of Crop Research,Fujian Academy of Agricultural Sciences/Fujian Province Characteristic Dry Crop Variety Breeding Engineering Technology Research Center,Fuzhou,Fujian 350013,China)

机构地区：[1]福建省农业科学院作物研究所/福建省特色旱作物品种选育工程技术研究中心,福建福州350013

出　　处：《福建农业学报》2022年第7期817-823,共7页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划公益类专项(2019R1031-13、2021R1031006);福建省种业创新与产业化工程项目(zycxny2021006);福建省科技计划重大专项(2020NZ08017);福建省农业高质量发展超越“5511”协同创新工程项目(XTCXGC2021019);福建省农业科学院科技创新团队建设项目(CXTD2021011-2)。

摘　　要：【目的】深入探究甜玉米甜度形成的关键基因及可能涉及的杂种优势分子机制。【方法】以超甜玉米品种闽双色6号及其双亲为研究材料,利用生物信息学,重测序,转录组等技术对蔗糖合酶基因家族进化、甜度相关基因分型及表达模式进行分析。【结果】玉米中存在18个蔗糖合酶基因;其中,Zm0001d029087与Sh1、Sus1和Sus2/3同属一个亚家族,进化上亲缘关系较近,表明Zm0001d029087可能在籽粒糖代谢中发挥功能;在双亲与B73的变异分析中发现,Sh1、Sus1和Sus2/3均存在突变,共同构成闽双色6号的超甜特性的遗传物质基础;表达分析发现,18个基因中仅Zm0001d029087与Sh1、Sus1和Sus2/3在籽粒中有表达。进一步对4个基因在闽双色6号及其双亲中的表达进行分析,结果显示,除Sh1基因外,其他3个基因在闽双色6号中的表达显著低于双亲或之一,表明闽双色6号在糖代谢途径上存在超亲遗传的分子机制。【结论】解析了闽双色6号籽粒超甜特性的遗传特性,发现1个潜在的功能基因Zm0001d029087,并初步探究杂种优势在闽双色6号超甜特性形成中的遗传机制。Objective This study explored the key genes of sweet corn sweetness formation and the possible molecular mechanism of heterosis.Method Genotypes and expressions of sucrose synthase gene family of a super-sweet corn variety,Minshuangse No.6,and its parents were analyzed using bioinformatics,resequencing,transcriptome,and other techniques to unveil the underlining basis of the sweet taste of corns.Result Eighteen sucrose synthase genes were isolated from the corn kernels.Among them,Zm0001d029087 was found to belong to the same subfamily as Sh1,Sus1,and Sus2/3 with close evolution relationships.It might play a role in the sugar metabolism of the corn.Comparing both parents with B73,mutations were found in Sh1,Sus1,and Sus2/3.Of the 18 genes,only Zm0001d029087 and those 4 genes expresses in the kernels.Further analyses showed the 4 genes,except Sh1,had significantly lower expressions in Minshuangse No.6 than in both or one of the parents.Conclusion The genetic variations in the super-sweet Minshuangse No.6 revealed a unique characteristic of Zm0001d029087 that might relate to the sweetness of the corn kernels.A preliminary role of heterosis in sugar formation by that gene was proposed for further investigation.

关 键 词：闽双色6号 甜度 遗传变异 表达分析 杂种优势 

分 类 号：S513[农业科学—作物学]