外壳蛋白ε在非小细胞肺癌中作用的探索  

Validation of the interaction between coatomer subunitεand sialylated immunoglobulin G after irradiation and exploration of coatomer subunitε’s role in non small cell lung cancer

作　　者：谷润川 杨雄涛 王国辉 许长丹 葛舒童 王昊[5] 高立伟[5] GU Run-chuan;YANG Xiong-tao;WANG Guo-hui(China-Japan Friendship Institute of Clinical Medicine,Beijing 100029,China)

机构地区：[1]中日友好临床医学研究所,北京100029 [2]昌平区医院肿瘤科,北京102200 [3]天津市第一中心医院放射治疗科,天津300100 [4]北京大学中日友好临床医学院,北京100029 [5]中日友好医院放射肿瘤科,北京100029

出　　处：《中日友好医院学报》2022年第5期272-277,F0002,共7页Journal of China-Japan Friendship Hospital

摘　　要：目的:对外壳蛋白ε与非小细胞肺癌的关系、唾液酸化免疫球蛋白G的结合及其与肺癌的关系进行探索。方法:通过细胞照射、免疫沉淀联合质谱分析研究唾液酸化免疫球蛋白与外壳蛋白ε的结合;依托TCGA、GEO、GTEx、HPA、EGA及KM plotter公共数据库数据,使用R对外壳蛋白ε与非小细胞肺癌的关联及机制进行分析。结果:外壳蛋白ε与唾液酸化免疫球蛋白G结合且在射线照射后升高,在非小细胞肺癌中高表达并与分期和不良预后相关,其与多种促肿瘤通路正相关并与多种抗肿瘤通路负相关。结论:外壳蛋白ε与唾液酸化免疫球蛋白相互作用,并在非小细胞肺癌中发挥促肿瘤效应。Objective:No systematic reports were found onassociation between coatomer subunitεand nonsmall cell lung cancer.This study explored further on binding of sialylated-immunoglobulin-G to coatomer subunitεand the role of coatomer subunitεin lung cancer.Methods:Binding of sialylated-immunoglobulinG to coatomer subunitεwas validated by immunoprecipitation and liquid mass spectrometry after irradiation.R was applied for analysis of associations between coatomer subunitεand non-small cell lung cancer,as well as the possible mechanisms underlying,with database TCGA,GEO,GTEx,HPA,EGA and KM plotter.Results:Coatomer subunitε,binding to sialylated immunoglobulin G and increasing after irradiation,was highly expressed in non-small cell lung cancer and was associated with staging and poor prognosis.Multiple tumour promoting pathways were found positively correlated with coatomer subunitεwhile multiple tumour suppressing pathways negatively correlated.Conclusion:Coatomer subunitεinteracts with sialylated-immunoglobulin-G and acts as a tumour promoting factor in non-small cell lung cancer.

关 键 词：唾液酸化免疫球蛋白G 外壳蛋白ε 非小细胞肺癌 生物信息学 

分 类 号：R734.2[医药卫生—肿瘤]