结直肠癌肝转移瘤远癌组织亚细胞结构与表达谱改变  

Transformation of Subcellular Structure and Expression Profile in Distant Cancerous Tissues of Liver Metastases from Colorectal Cancer

作　　者：蓝淞耀 龚晗 韦成江 唐琪[1] 冯雁[1] 林源[1] LAN Songyao;GONG Han;WEI Chengjiang(The Affiliated Tumor Hospital,Guangxi Medical University,Guangxi Nanning 530021,China)

机构地区：[1]广西医科大学附属肿瘤医院胃肠外科结直肠肛门病区,广西南宁530021

出　　处：《河北医学》2022年第10期1609-1614,共6页Hebei Medicine

基　　金：广西壮族自治区科学技术厅广西重点研发项目,(编号:桂科AB16380202);广西医科大学大学生创新创业训练计划项目,(编号:202110598004)。

摘　　要：目的:探讨结直肠癌肝转移瘤中肝脏远癌组织的亚细胞结构与表达谱改变情况。方法:电镜观察人体肝脏远癌组织亚细胞结构变化,通过建立动物结直肠癌肝转移模型观察小鼠远癌肝组织亚细胞结构变化;利用转录组测序探究动物模型中肝脏远癌组织的表达谱改变。结果:人与小鼠肝转移组织中的亚细胞结构均发生异于正常组织的改变。转录组分析结果显示在远癌组织中上调基因的表达和富集通路均与肿瘤发生发展相关。结论:远癌组织的形态结构与基因表达发生类似于肿瘤组织的改变。Objective:To investigate the changes of subcellular structure and expression profile of distal tissue in colorectal cancer liver metastasis.Methods:The structural changes of human liver were observed by electron microscope,and confirmed in a colorectal cancer liver metastasis mice model.Transcriptome profiling was used to explore the differential expressed genes in distal tissues in the mice model.Results:The subcellular structure of liver cells in distant tissue in both human and mouse liver metastases were obviously different from that of healthy liver cells.Transcriptome analysis showed that differentiated genes in distal tissues were enriched to pathways related to tumor.Conclusion:The morphological structure and gene expression of distal tissues were also affected by the liver metastasis.

关 键 词：结直肠癌 肝转移 细胞亚结构 远癌组织 

分 类 号：R735.34[医药卫生—肿瘤]