Smurf participates in Helicoverpa armigera diapause by regulating the transforming growth factor-βsignaling pathway  

作　　者：Haiyin Li Qin Lu Yan Li Yufang Yan Zhiyong Yin Jianjun Guo Weihua Xu 

机构地区：[1]Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region,Institute of Entomology,Guizhou University,Guiyang,China [2]State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-sen University,Guangzhou,China

出　　处：《Insect Science》2022年第5期1251-1261,共11页昆虫科学（英文版）

基　　金：supported by funds from the Technol-ogy Project of Guizhou Province(Qian Ke He Basic-ZK[2021]General 102);Youth Talents of Science and Tech-nology Projects from Guizhou Educational Department(Qian Jiao He KY Zi[2021]078);Talent Introduction Project of Guizhou University(Gui Da Ren Ji He[2019]04).

摘　　要：Diapause,an important strategy used by insects to avoid adverse environments,is regulated by various cell signaling pathways.The results of our previous studies demonstrated that the transforming growth factor-β(TGF-β)signaling pathway regulated pupal diapause in Helicoverpa armigera,which was accompanied by downregulation of proteins in TGF-βsignaling.However,to date the mechanism underlying this phenomenon remains unknown.Here,we cloned the E3 ubiquitin ligases gene Smurf.In vitro experiments showed that Smurf directly bound to TGF-βreceptor type I(TGFβRI)and Smad2.Overexpressing Smurf promoted ubiquitination of TGFβRI and Smad2,thereby downregulating their protein levels.Conversely,silencing of the Smurf gene suppressed ubiquitination of TGFβRI and Smad2 thereby increasing their protein levels.Results from in vivo co-immunoprecipitation assays revealed that the binding of Smurf to TGFβRI or Smad2 was stronger in diapause pupae than in nondiapause pupae.Injection of Smurf inhibitor A01 into diapause pupae markedly upregulated expression of TGFβRI and Smad2 proteins,leading to resumption of development in diapause pupae.Taken together,these findings suggested that ubiquitin ligase E3 Smurf participated in H.armigera diapause by regulating TGF-βsignaling,and thus could be playing a crucial role in insect diapause.

关 键 词：degradation DIAPAUSE Helicoverpa armigera Smurf TGF-βsignaling UBIQUITINATION 

分 类 号：Q96[生物学—昆虫学]