Functional characterization of a low-density lipoprotein receptor in the lepidopteran model,Bombyx mori  

作　　者：Gui-Yun Li Xiao-Jing Liu Gang-Qi Fang Jie Yang Shuai Zhan Mu-Wang Li 

机构地区：[1]Jiangsu Key Laboratory of Sericultural Biology and Biotechnology,School of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang,Jiangsu,China [2]CAS Key Laboratory of Insect Developmental and Evolutionary Biology,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai,China [3]The Key Laboratory of Silkworm and Mulberry Genetic Improvement,Ministry of Agriculture,Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang,Jiangsu,China [4]CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing,China

出　　处：《Insect Science》2022年第5期1262-1274,共13页昆虫科学（英文版）

基　　金：supported by the National Science Foundation of China(grant numbers 32021001 and 32000339);Postgraduate Research&Practice Innovation Program of Jiangsu Province.

摘　　要：The growth and development of metabolous insects are mainly regulated by ecdysone and juvenile hormone.As a member of the low-density lipoprotein receptor(LDLR)family,megalin(mgl)is involved in the lipoprotein transport of cholesterol which is an essential precursor for the synthesis of ecdysone.Despite extensive studies in mammals,the function of mgl is still largely unknown in insects.In this study,we characterize the function of mgl in the silkworm Bombyx mori,the model species of Lepidoptera.We find that mgl is broadly present in the genomes of lepidopteran species and evolved with divergence between lepidopterans and Drosophila.The expression pattern suggests a ubiquitous role of mgl in the growth and development in the silkworm.We further perform clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9-based mutagenesis of Bmmgl and find that both the development and the silk production of the silkworm are seriously affected by the disruption of Bmmgl.Our results not only explore the function of mgl in Lepidoptera but also add to our understanding of how cholesterol metabolism is involved in the development of insects.

关 键 词：Bombyx mori CHOLESTEROL CRISPR/Cas9 development MEGALIN SILKWORM 

分 类 号：Q96[生物学—昆虫学]