检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:黄明珠 姚坤 宋卓琳 张豪 刘斌 陈雪岚 HUANG Ming-zhu;YAO Kun;SONG Zhuo-lin;ZHANG Hao;LIU Bin;CHEN Xue-lan(National R&D Center for Freshwater Fish Processing,Nanchang 330022,China;College of Life Sciences,Jiangxi Normal University,Nanchang 330022,China)
机构地区:[1]国家淡水鱼加工技术研发专业中心,南昌330022 [2]江西师范大学生命科学学院,南昌330022
出 处:《中国生物工程杂志》2022年第9期105-115,共11页China Biotechnology
基 金:国家自然科学基金(31960014、31660019);江西省自然科学基金(20202ACBL205001);江西省教育厅科技项目(GJJ210321)资助项目。
摘 要:人类社会工业化导致各种有毒物质被排放到环境中造成严重的污染。除了自然降解外,传统的处理方法包括化学转化、物理吸附、离子交换和电化学方法等,但存在二次污染、能源需求高、投资成本高、再生效率低、低浓度废水处理效率低等缺点。细胞表面展示技术是一种通过表面锚定蛋白在细胞表面连接功能肽的新型、高效的生物技术。与细胞内和分泌物表达系统相比,微生物表面展示的蛋白质对有机溶剂、蛋白酶、温度和pH的变化表现出更强的稳定性。通过细胞培养就可以获得固定在细胞表面的蛋白酶,避免了蛋白质纯化、浓缩等繁琐的程序。此外,细胞表面展示技术是良好的单细胞水平突变体文库高通量筛选平台。综述细胞表面展示技术在环境生物修复方面的研究进展,重点介绍该技术的应用和未来发展前景。Industrialization has led to various toxic compounds emission, which causes environmental contamination. Aside from natural geological weathering, traditional solutions include chemical conversion, physical absorption, electrochemical methods and ions exchange, but their applications are limited due to some disadvantages, such as secondary pollution, high energy requirement, high investment cost, low regeneration efficiency and inefficiency in low-concentration wastewater treatment. Cell surface engineering is an innovative, cost-effective biotechnology of microorganism for modification of cell surface function through joining external functional peptides with surface anchoring proteins. In contrast to conventional intracellular and secretion expression systems, proteins displayed on the surface of microorganism may exhibit enhanced stability against changes in organic solvents, proteases, temperature and pH. Surface-engineered cells prepared by cultivation are ready to be used as microparticles covered with proteins/peptides, avoiding troublesome concentration procedures and protein purification. Furthermore, cell-surface display engineering is suitable for high throughput screening from the mutant library for more capable proteins/peptides at the single-cell level. Currently, this technology is widely used in the control of environmental pollution. This review is focused on recent strategies of using cell-surface display technology in environmental bioremediation, summarizing its applications, recent progress and future prospects.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.200