文山红河地区苗族人群20个常染色体STR基因座遗传多态性研究  

Genetic polymorphisms of 20 Autosomal STR loci in Miao Population from Wenshan and Honghe Regions,Yunnan Province

作　　者：徐冲冲 王上 魏巍 张柠 黎宽 连心情 王诗旭 周雪梅 卢晓筱 钟树荣 XU Chongchong;WANG Shang;WEI Wei;ZHANG Ning;LI Kuan;LIAN Xinqing;WANG Shixu;ZHOU Xuemei;LU Xiaoxiao;ZHONG Shurong(School of Forensic Medicine,Kunming Medical University,Kunming Yunnan 650500;The Forensic Medical Center of Beijing Municipal Public Security Bureau,Beijing 100192;Beijing Tongda Shoucheng Institute of Forensic Science,Beijing 100089;Judicial Identification Center of Kunming Medical University,Kunming Yunnan 650500,China)

机构地区：[1]昆明医科大学法医学院,云南昆明650500 [2]北京市公安局法医中心,北京100192 [3]北京通达首诚司法鉴定,北京100089 [4]昆明医科大学司法鉴定中心,云南昆明650500

出　　处：《昆明医科大学学报》2022年第10期153-162,共10页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81660232,81000577);云南省科技厅生物医药领域重大专项基金资助项目(2019ZF003);昆明医科大学百名中青年学术技术骨干基金资助项目(60117190413)。

摘　　要：目的研究文山红河地区苗族群体20个常染色体STR基因座的遗传多态性,评估其在该人群中的法医学应用价值,为法医DNA分析和人类遗传研究提供基础数据。方法采用Chelex-100法提取样本DNA,使用PowerPlex~?21试剂盒对该地区2209例苗族无关健康个体STR基因座进行复合扩增,用ABI 3130自动遗传分析仪对扩增产物进行毛细管电泳分析,用Genemapper ID v3.2软件进行基因分型。采用Modified-Powerstats软件进行Hardy-Weinberg平衡检验,并计算法医学参数。应用Arlequin和Phylip软件分别计算Fst值和Nei’s遗传距离。使用SPSS软件进行多维尺度(MDS)分析。采用Mega软件构建相邻连接(N-J)系统发育树。结果20个常染色体STR基因座共检出265个等位基因和1080种基因型。等位基因频率分布为0.0002~0.5718,各基因座等位基因频率和基因型频率分布均符合Hardy-Weinberg平衡(P>0.05/20=0.0025)。累积非父排除率(CPE)为0.999999935545335,累积个人识别概率(CDP)为0.999999999999999999999932674151。Nei’s遗传距离、N-J系统发育树和MDS分析显示,文山红河地区苗族与云南越南人群遗传关系最近。结论20个常染色体STR基因座在文山红河地区苗族群体中具有较高的遗传多态性,可用于法医学鉴定和群体遗传学研究。Objective To investigate the genetic polymorphisms of 20 autosomal STR loci in Miao population from Wenshan and Honghe regions,and to explore their forensic values.Methods DNA was extracted with the Chelex-100 method.A total of 20 STR loci were amplified from 2209 healthy unrelated individuals of the Miao population from Wenshan and Honghe regions,Yunnan Province using the PowerPlex21 System kit.The PCR products were separated by capillary electrophoresis on an ABI 3130Genetic Analyzer,and alleles were genotyped by GeneMapper ID(Version 3.2)software.Forensic parameters were calculated by Modified-Powerstats software,and the Hardy-Weinberg equilibrium(HWE)test was performed.The Fst values and Nei’s genetic distance were calculated by Arlequin and Phylip software,respectively.Multidimensional scaling(MDS)analysis was performed with SPSS v24.0 statistical software and the neighbor-joining(N-J)phylogenetic tree was constructed using the MEGA v6.0 software.Results A total of 265 alleles and 1080 genotypes were observed.The allele frequency of the 20 STRs ranged from 0.0002 to 0.5718.No deviation from Hardy-Weinberg equilibrium(P>0.05/20=0.0025)was observed.The cumulative discrimination power(CDP)and cumulative probability of exclusion(CPE)with 20 STRs were 0.999999999999999999999932674151 and 0.999999935545335,respectively.Population comparison showed that Wenshan and Honghe Miao ethnic group was genetically closer to Yunnan Vietnamese.Conclusion These 23 STR loci are highly genetic polymorphic and informative in the Miao population from Wenshan and Honghe regions,Yunnan Province,which can be used for forensic identification and population genetic research.

关 键 词：法医遗传学 遗传多态性 短串联重复 文山红河苗族 

分 类 号：R394[医药卫生—医学遗传学]