钙黏蛋白5驱动内皮细胞特异性过表达Dickkopf 1影响骨形成  

Endothelial-cell-specific overexpression of Dickkopf 1 using Cadherin 5 promoter regulates osteogenesis in vivo

作　　者：尹一佳 杨瑾廷 申建琪 黄凌依 井岩 官秋玥[4] 韩向龙[1] Yin Yijia;Yang Jinting;Shen Jianqi;Huang Lingyi;Jing Yan;Guan Qiuyue;Han Xianglong(State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Dept.of Orthodontics,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China;Fenyang College of Shanxi Medical University,Lüliang 032299,China;Baylor College of Dentistry,Texas A&M University,Dallas TX 75246,USA;Geriatric Department,Caotang District,Sichuan Provincial People’s Hospital,Chengdu 610072,China)

机构地区：[1]口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院正畸科,成都610041 [2]山西医科大学汾阳学院,吕梁032299 [3]美国德克萨斯州农工大学贝勒牙科学院,达拉斯TX 75246 [4]四川省人民医院草堂病区老年病房,成都610072

出　　处：《国际口腔医学杂志》2022年第6期641-647,共7页International Journal of Stomatology

基　　金：四川省科技厅应用基础研究项目(2018JY0271)。

摘　　要：目的通过检测体内内皮细胞特异性过表达Dickkopf 1(DKK1)对股骨和牙槽骨骨形成和血管形成的影响,为DDK1在正畸牙移动骨重建和血管形成中的作用提供依据。方法构建钙黏蛋白5(Cdh5)驱动的内皮细胞特异性DKK1过表达转基因小鼠(Cdh5-Cre;R26-DKK1/R26-DKK1),检测股骨和牙槽骨骨形态和骨量的变化。结果Cdh5-Cre;R26-DKK1/R26-DKK1小鼠的股骨和牙槽骨DKK1高表达,骨量增加,骨髓腔缩窄;股骨中Trap+细胞数目降低;股骨和牙槽骨中Ⅳ型胶原蛋白表达增加。结论DKK1可能调控正畸牙移动。Cdh5驱动内皮细胞中DKK1表达增加与骨组织中软骨细胞的分化及骨小梁的形成、牙周组织骨量的增加密切相关。Objective This study aimed to the expression of Dickkopf 1(DKK1)during orthodontic tooth movement is examined.Then,the effects of endothelial-cell-specific overexpression of DKK1 on bone formation were detected.Moreover,the function of DKK1 on bone formation through endothelial cells was explained.Methods A rat orthodontic tooth movement model was constructed to detect the expression of DKK1 on the compressive and tensile side of alveolar bone.Then,R26-DKK1 and Cadherin 5(Cdh5)-Cre mice were crossbred to produce transgenic mice expressing DKK1 in endothelial cells(Cdh5-Cre;R26-DKK1/R26-DKK1)and to detect the changes of bone morphology and bone volume of the femur and alveolar bone.Results During orthodontic tooth movement,DKK1 was highly expressed in the compres‐sive side.DKK1 transgenic mice widely expressed DKK1 in the femur and alveolar bone,increased in bone density,narrowed in central bone marrow cavity,and in‐creased in Trapcells numbers and collagenⅣexpres‐sion compared with wild type controls.Conclusion DKK1 might regulate orthodontic tooth movement.Endothelial-cell-specific overexpression of DKK1 using Cdh5 pro‐moter is closely related to the differentiation of chondrocytes and the formation of trabecular bone,and causes increased expression level of collagenⅣin periodontal tissue and bone.

关 键 词：内皮细胞 骨形成 股骨 牙槽骨 

分 类 号：R782[医药卫生—口腔医学]