羟基红花黄色素A促进大鼠随意型皮瓣存活  

Hydroxysafflor yellow A could promote the survival of random skin flaps in rats

作　　者：李晓璐 李羡 陈聪 陈元[2] 王一帆 李笑寒 胡勇[1] 李秀存 Li Xiaolu;Li Xian;Chen Cong;Chen Yuan;Wang Yifan;Li Xiaohan;Hu Yong;Li Xiucun(Department of Hand and Foot Surgery,the Second Hospital of Shandong University,Jinan 250012,China;Institute of Medical Sciences,the Second Hospital of Shandong University,Jinan 250012,China)

机构地区：[1]山东大学第二医院手足外科,济南250012 [2]山东大学第二医院基础医学研究所,济南250012

出　　处：《中华整形外科杂志》2022年第9期1041-1046,共6页Chinese Journal of Plastic Surgery

基　　金：山东省自然科学基金(ZR2020MH195)。

摘　　要：目的探讨羟基红花黄色素A(HSYA)对大鼠随意型皮瓣存活的影响。方法30只SD大鼠采用随机数字表法分为HSYA组和生理盐水(NS)组,每组15只。采用改良的McFarlane皮瓣模型,于大鼠背部正中切取3 cm×12 cm矩形随意型皮瓣并原位缝合,皮瓣自尾端向头侧平均分为4等份,依次标记为Ⅰ~Ⅳ区。HSYA组将注射用HSYA以0.9%氯化钠溶液溶解后立即于大鼠腹腔注射(20 mg/kg),NS组腹腔注射同等体积的生理盐水,每日1次,持续14 d。术后第14天,对大鼠皮瓣进行拍照用于评估皮瓣成活面积百分比,同时,在大鼠皮瓣Ⅲ区进行取材,用于组织学分析(HE染色分析真皮下层直径>0.1 mm的血管数目,CD31免疫组织化学染色分析真皮下层微血管密度),以及实时荧光定量PCR[检测内皮型一氧化氮合酶(eNOS)和血管内皮细胞生长因子受体2(VEGFR2)mRNA的表达]。数据以x±s表示,2组间比较采用独立样本t检验。结果(1)HSYA组的皮瓣成活面积百分比明显高于NS组(70.4%±7.0%vs.55.4%±7.7%,P<0.01)。(2)HSYA组直径>0.1 mm血管的数目明显多于NS组[(31.5±5.0)条vs.(15.3±3.4)条,P<0.01]。(3)HSYA组微血管密度明显高于NS组[(82.8±14.0)条/mm^(2) vs.(43.0±4.6)条/mm^(2),P<0.01]。(4)HSYA组的eNOS和VEGFR2 mRNA相对表达量均明显高于NS组(3.0±0.9 vs.1.2±0.8;14.2±7.7 vs.1.1±0.6;均P<0.05)。结论HSYA可能通过上调eNOS和VEGFR2的基因表达,促进随意型皮瓣内的血管扩张和微血管生成,从而增加随意型皮瓣的成活面积。Objective To explore the effect of hydroxysafflor yellow A(HSYA)on the viability of the random skin flap in rats.Methods A total of 30 SD rats were randomly divided into HSYA group and normal saline(NS)group.Using the modified McFarlane skin flap model,a rectangular random skin flap of approximately 3 cm×12 cm was dissected and sutured in situ over the central dorsum of the rats.The flap was divided into four zones from its caudal part to the cranial part:ZoneⅠ,ZoneⅡ,ZoneⅢ,and ZoneⅣ.The rats in the HSYA group received intraperitoneal injection of HSYA(20 mg/kg)dissolved in 0.9%sodium chloride solution immediately,while NS group rats were given intraperitoneal injection of an equal amount of NS.The injection was performed once a day for 14 days.On postoperative day 14,the flap was photographed to evaluate the survival rate.Tissue samples were collected from ZoneⅢof the flaps for histological analysis and real-time quantitative PCR(RT-qPCR).The number of the vessels with a diameter greater than 0.1 mm in the subdermal layer was evaluated using hemotoxylin&eosin(HE)staining.Microvascular density in the subdermis was assessed by the immunohistochemical(IHC)staining of CD31.The gene expression levels of endothelial nitric oxide synthase(eNOS)and vascular endothelial growth factor receptor 2(VEGFR2)was quantified with RT-qPCR.Results The mean survival rate of HSYA group(70.4%±7.0%)was significantly higher than that of NS group(55.4%±7.7%,P<0.01).The number of blood vessels>0.1 mm in the HSYA group(31.5±5.0)was significantly higher than that in the NS group(15.3±3.4,all P<0.01).The mean microvascular density in HSYA group(82.8±14.0/mm^(2))was significantly greater than that in NS group(43.0±4.6/mm^(2),P<0.01).Compared with NS group,the mRNA expression of eNOS and VEGFR2 was upregulated in HSYA group(3.0±0.9 vs.1.2±0.8;14.2±7.7 vs.1.1±0.6;P<0.05).Conclusions HSYA might promote the vasodilation and angiogenesis through up-regulating eNOS and VEGFR2 expression,thus increasing the viability of the rando

关 键 词：羟基红花黄色素A 随意型皮瓣 血管扩张 血管生成 

分 类 号：R622[医药卫生—整形外科]