环状RNA m6A甲基化修饰在口腔疾病中的研究进展  

Research progress on m6A⁃modified circRNA in oral diseases

作　　者：杨靖雯 周海文[1] YANG Jingwen;ZHOU Haiwen(Department of oral mucosal diseases,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong UniversityNational center for Stomatoloy,National Clinical Research Center for oral diseases,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology,Shanghai 200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院口腔黏膜病科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海200011

出　　处：《口腔疾病防治》2023年第2期137-141,共5页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：国家自然科学基金项目(81170968)。

摘　　要：N6⁃甲基腺苷(N6⁃methyladenosine,m6A)修饰是真核生物最丰富的一种RNA修饰,广泛参与RNA的核输出、剪接、翻译和降解等过程的调控。越来越多的证据表明环状RNA(circular RNA,circRNA)m6A甲基化修饰与代谢、免疫以及良恶性疾病有关。本文对circRNA m6A甲基化修饰在机体生理过程、恶性肿瘤中的作用及口腔疾病中的研究现状作一综述。现有研究显示,m6A甲基化修饰主要通过调节circRNA翻译、促进circRNA的核输出以及促进circRNA降解对circRNA产生影响;circRNA m6A甲基化修饰在免疫、生殖系统、肌细胞发生发育和恶性肿瘤中发挥调控作用;m6A甲基转移酶蛋白3(methyltransferase⁃like 3,METTL3)通过YTH m6A RNA结合蛋白1(YTH m6A RNA binding protein 1,YTHDF1)介导的m6A修饰促进口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)的发生,METTL14的高表达可有效降低OSCC的增殖、迁移和侵袭;circRNA m6A甲基化修饰在口腔领域的研究较少,仅限于成釉细胞瘤和口腔种植骨吸收方向的初步研究,在口腔重大疾病(如口腔潜在恶性疾病及OSCC)方向有广阔的研究前景。N6⁃methyladenosine(m6A)modification is the most abundant RNA modification in eukaryotes and is widely involved in the regulation of RNA nuclear export,splicing,translation and degradation.Increasing evidence shows that m6A methylation modification of circular RNA(circRNA)has great potential in metabolism,immunity and be⁃nign and malignant diseases.Here,we review the research status of circRNA m6A methylation modification in physiolog⁃ical processes,malignant tumors and oral diseases.It has been shown that m6A methylation can regulate circRNA by reg⁃ulating circRNA translation,promoting circRNA nuclear output,and promoting circRNA degradation;M6A⁃modified cir⁃cRNA plays a regulatory role in immunity,the reproductive system,myogenesis and development,and malignant tumors;and the M6A methyltransferase METTL3 promotes the occurrence of oral squamous cell carcinoma(OSCC)through m6A modification mediated by YTHDF1.The high expression of METTL14 can effectively reduce the proliferation,migration and invasion of OSCC.There is little research on circRNA m6A methylation modification in the oral cavity,which is limited to preliminary research in ameloblastoma and oral implant bone absorption.It has broad research prospects in the treatment of major oral diseases(such as potential oral malignant diseases and OSCC).

关 键 词：环状RNA 非编码RNA 甲基化修饰 N6⁃甲基腺苷 口腔鳞状细胞癌 口腔潜在恶性疾病 口腔 肿瘤 生物标志物 

分 类 号：R78[医药卫生—口腔医学]