miR-221/222与乳腺癌ERα表达及他莫昔芬敏感性的关系  

Relationship between Mir-221/222 and ERα expression and tamoxifen sensitivity in breast cancer

作　　者：侯蕾 马源婧 霍欣欣 史学丽 HOU Lei;MA Yuanjing;HUO Xinxin;SHI Xueli(Department of Maternal and Child Health Care,Shijiazhuang Maternal and Child Health Care Hospital,Shijiazhuang,Hebei,China,050011;Department of Laboratory Medicine,Shijiazhuang Maternal and Child Health Hospital,Shijiazhuang,Hebei,China,050011;Department of Radiology,Shijiazhuang Maternal and Child Health Care Hospital,Shijiazhuang,Hebei,China,050011;Department of Clinical Pharmacy,Shijiazhuang Maternal and Child Health Hospital,Shijiazhuang,China Hebei,050011)

机构地区：[1]河北省石家庄市妇幼保健院孕产保健部,河北石家庄050011 [2]河北省石家庄市妇幼保健院检验科,河北石家庄050011 [3]河北省石家庄市妇幼保健院放射科,河北石家庄050011 [4]石家庄市妇幼保健院临床药学研究室,河北石家庄050011

出　　处：《分子诊断与治疗杂志》2022年第10期1667-1671,共5页Journal of Molecular Diagnostics and Therapy

基　　金：河北省医学科学研究课题计划(20210699)。

摘　　要：目的 探讨miR-221/222与乳腺癌临床病理的关系,及是否参与ERα的调节并影响他莫昔芬(TAM)的敏感性。方法 分析2019年1月至2021年12月石家庄市妇幼保健院经手术切除的104例乳腺浸润性导管癌及其癌旁组织标本,采用茎环引物实时荧光定量PCR(qPCR)法检测癌组织和相应癌旁组织中miR-221/222的表达水平,采用免疫组织化学法检测ERα表达,分析miR-221/222与ERα表达的关系。MCF-7细胞转染空白试剂、miR-221抑制剂、miR-222抑制剂和miR-221/222抑制剂,转染后均给予TAM 5μg/mL进行培养并检测其细胞增殖抑制率和细胞凋亡率。结果 乳腺癌组织中的miR-221/222表达明显高于癌旁组织,差异具有统计学意义(t=17.541,P<0.05;t=12.911,P<0.05);miR-221/222的表达水平在ER-α表达阴性、PR表达阴性组均显著高于阳性表达组(t=7.039,P<0.05;t=5.357,P<0.05),miR-221/222的表达水平在Her-2表达阳性显著高于阴性组(t=9.827,P<0.05);在三阴性分子亚型中的表达显著高于Her-2过表达型、luminal A及luminal B1型乳腺癌(F=23.127,P<0.05)。miR-221/222抑制组转染后24、48 h细胞增殖抑制率、细胞凋亡率均明显高于空白对照组、miR-221抑制组、miR-222抑制组(F=19.736,P<0.05;F=36.091,P<0.05)。结论 miR-221/222水平与腺癌患者的ERα表达相关,且miR-221/222可影响乳腺癌细胞对TAM的敏感性。Objective To investigate the relationship between Mir-221/222 and clinicopathology of breast cancer,and whether it is involved in the regulation of ERα and affects the sensitivity of tamoxifen(TAM). Methods A retrospective analysis was performed on 104 cases of invasive ductal carcinoma of breast and its adjacent tissues surgically resected from January 2019 to December 2021 in Shijiazhuang Maternal and Child Health Care Hospital. The expression levels of Mir-221/222 in the carcinoma tissues and corresponding adjacent tissues were detected by real-time fluorescence quantitative PCR(qPCR)with stem ring primers. The expression of ERα was detected by immunohistochemistry,and the relationship between Mir-221/222 and ERαexpression was analyzed. MCF-7 cells were transfected with blank reagent,Mir-221 inhibitor,Mir-222inhibitor and Mir-221/222 inhibitor. After transfection,all MCF-7 cells were treated with TAM 5 μg/m L for culture and proliferation inhibition rate and apoptosis rate were detected. Results The expression of Mir-221/222 in breast cancer tissues was significantly higher than that in adjacent tissues(t=17.541,P<0.05;t=12.911,P<0.05). The expression level of Mir-221/222 in ER-α negative group and PR negative group was significantly higher than that in the positive group(t=7.039,P<0.05;t=5.357,P<0.05). The expression level of Mir-221/222 in HER-2 positive group was significantly higher than that in the negative group(t=9.827,P<0.05). The expression of triple negative subtype was significantly higher than that of HER-2 overexpression,Luminal A and Luminal B1 breast cancer(F=23.127,P<0.05). The cell proliferation inhibition rate and cell apoptosis rate of Mir-221/222 inhibition group at 24 and 48 h after transfection were significantly higher than those of blank control group,Mir-221 inhibition group and Mir-222 inhibition group(F=19.736,P<0.05;F=36.091,P<0.05). Conclusion The level of Mir-221/222 is correlated with the expression of ERα in patients with adenocarcinoma,and Mir-221/222 could affect the sen

关 键 词：乳腺癌 雌激素受体-Α RNA 他莫昔芬 敏感性 

分 类 号：R737.9[医药卫生—肿瘤]