EB-DNA检测在重离子治疗鼻咽癌中的临床应用价值  

Clinical application of EB-DNA detection in heavy ion therapy for nasopharyngeal carcinoma

作　　者：王兴昌 陈东基 周俊年 李霞 杨婷 WANG Xingchang;CHEN Dongji;ZHOU Junnian;LI Xia;YANG Ting(Department of Laboratory Medicine,Wuwei Tumor Hospital,Wuwei,Gansu,China,733000)

机构地区：[1]甘肃省武威肿瘤医院检验科,甘肃武威733000

出　　处：《分子诊断与治疗杂志》2022年第10期1792-1795,共4页Journal of Molecular Diagnostics and Therapy

摘　　要：目的 探讨血浆EB病毒(EB)-DNA检测在重离子治疗鼻咽癌(NPC)中的临床应用价值。方法 选取2021年8月至2022年5月期间甘肃省武威肿瘤医院收治的42例鼻咽癌患者资料进行分析并作为病例组,选取同期门诊体检30名健康志愿者作为对照组。病例组患者均接受重离子放射治疗。检测对照组、病例组(治疗前和治疗1个月后)的血浆EB-DNA的表达情况,比较对照组与病例组治疗前EB-DNA阳性检出率。以鼻咽部活检诊断为诊断金标准,绘制ROC曲线对治疗前EB-DNA检测结果对NPC诊断效能进行分析。治疗后1个月对患者进行EB-DNA检测,根据结果将患者分为阴性组与阳性组,比较两组的近期疗效。结果 治疗前,病例组EB-DNA拷贝量显著高于对照组,EB-DNA阳性检出率低于对照组,差异有统计学意义(u=6.659、H=44.345,P均<0.05);血浆EB-DNA表达对NPC诊断的ROC曲线下面积(AUC)为0.963[P<0.05,95%CI(0.916~1.000)],敏感度、特异度分别为0.929、0.933;治疗后1个月EB-DNA检测,阴性组30例,阳性组12例,阴性组近优于阳性组,差异有统计学意义(H=5.040,P<0.05)。结论 EB-DNA检测可作为NPC早期诊断与重离子放射治疗疗效和预后的参考指标。Objective To explore the clinical application of plasma Epstein Barr virus(EB)-DNA detection in heavy ion therapy for nasopharyngeal carcinoma. Methods The data of 42 NPC patients who were admitted to Wuwei Tumor Hospital of Gansu Province between August 2021 and May 2022 were retrospectively reviewed and used as the case group,and 30 healthy volunteers who were examined in outpatient clinics during the same period were selected as the control group. Patients in the case group were all treated with heavy ion radiotherapy. The expression of plasma EB-DNA in the control group and the case group(before treatment and 1month after treatment)was examined,and the positive detection rate of EB-DNA in the control group was compared with that in the case group before treatment. The diagnostic efficacy of pretreatment EB-DNA test results for NPC was analyzed by plotting the ROC curve,using nasopharynx biopsy as the diagnostic gold standard. EB-DNA testing was performed on patients 1 month after treatment,and the patients were divided into the negative group versus the positive group according to the results,and the short-term curative effects of the two groups were compared. Results Before treatment,the copy number of EB-DNA in the case group was significantly higher than that in the control group,and the positive detection rate of EB-DNA in the case group was lower than that in the control group,and the difference was statistically significant(u=6.659,H=44.345,both P<0.05). The area under the ROC curve(AUC)of plasma EB-DNA expression for NPC diagnosis was0.963[P<0.05,95% CI(0.916-1.000)],and the sensitivity and specificity were 0.929,0.933. One month after treatment,EB-DNA test showed that there were 30 cases in the negative group and 12 cases in the positive group.The negative group was nearly better than the positive group,and the difference was statistically significant(H=5.040,P<0.05). Conclusion EB-DNA detection can be used as a reference indicator for early diagnosis of nasopharyngeal carcinoma in combination with he

关 键 词：EB-DNA 诊断 重离子治疗 鼻咽癌 疗效 

分 类 号：R739.63[医药卫生—肿瘤]