便携式微器件偶联质谱技术在生物检测中的研究进展  

Advances in Portable Microdevice Coupled Mass Spectrometry for Biological Detection

作　　者：王晨 李恺 闵乾昊 WANG Chen;LI Kai;MIN Qian-Hao(State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023,China)

机构地区：[1]南京大学化学化工学院,生命分析化学国家重点实验室,南京210023

出　　处：《分析化学》2022年第11期1685-1698,共14页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21974062,92053102)资助。

摘　　要：便携式微器件偶联质谱技术在确保质谱检测灵敏度和稳定性的同时,通过简化样品预处理步骤可实现生物样本的快速采样与高通量检测。纸基材料、微萃取器件和微芯片等技术将快速采样、除盐和萃取功能集成一体,与上述器件联用的质谱分析技术在生物检测方面表现出了良好的灵敏度和选择性,已被广泛应用于药物监测、内源性小分子检测、蛋白质分析和细菌捕获分析等领域。本文总结了以纸基电喷雾-质谱、微萃取-质谱、微芯片-质谱为代表的便携式微器件偶联质谱技术的主要原理和研究进展,并展望了其未来发展趋势及应用前景。Portable microdevice coupled mass spectrometry(MS) has shown great capability of rapid sampling and high-throughput assay of biological samples by simplifying sample pretreatment while maintaining the sensitivity and reliability of MS detection. Particularly, paper substrates, microextraction devices and microchips allow the integration of fast sampling, desalting and extraction. Mass spectrometric methods coupled with above portable microdevices show great sensitivity and selectivity in biological detections, thus facilitating their applications in therapeutic drug monitoring, endogenous substrate detection, protein and peptide detection, and bacterial identification. This review summarizes the main principles and research progress of portable microdevice coupled mass spectrometry(e.g. paper spray-MS, microextraction-MS, and microfluidic chip-MS), and also prospects their future developments and potential applications.

关 键 词：便携式微器件 质谱 纸基电喷雾 微萃取 微芯片 评述 

分 类 号：O657.63[理学—分析化学] Q503[理学—化学]