以毛细管液柱高度为读出信号的便携式硫化氢传感器的构建与应用  

Construction and Application of Portable Hydrogen Sulfide Sensor with Capillary Liquid Column Height as Readout

作　　者：李志新 陈舒婷 罗芳 林振宇 LI Zhi-Xin;CHEN Shu-Ting;LUO Fang;LIN Zhen-Yu(Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety,Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology,College of Chemistry,Fuzhou University,Fuzhou 350116,China;College of Biological Science and Engineering,Fuzhou University,Fuzhou 350116,China)

机构地区：[1]福州大学化学学院,食品安全与生物分析教育部重点实验室,福建省食品安全分析与检测重点实验室,福州350116 [2]福州大学生物科学与工程学院,福州350116

出　　处：《分析化学》2022年第11期1733-1742,共10页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21775026)资助。

摘　　要：采用毛细管液柱高度作为读出信号,构建了一种检测硫化氢(H_(2) S)的便携式传感器。修饰有叠氮基的磁珠(MBs-N_(3))与修饰有炔基的铂纳米粒子(Pt NPs-Alk)在Cu^(+)的催化作用下发生点击化学反应,形成表面连接铂纳米粒子的磁珠(MBs-Pt NPs)。MBs-Pt NPs经磁分离后,转移至装有过氧化氢(H_(2) O_(2))溶液且瓶口组装有毛细管的玻璃瓶中,MBs-Pt NPs表面的铂纳米粒子可通过催化H_(2) O_(2)分解产生大量的氧气,从而导致该封闭反应装置中压力增大,毛细管内液柱上升。当体系中存在H_(2) S时,由于MBs-N_(3)表面的叠氮基可被H_(2) S还原为氨基,减少了其与Pt NPs-Alk之间的点击化学反应位点,磁珠表面的铂纳米粒子数量减少,催化H_(2) O_(2)分解的效率降低,导致毛细管液柱上升高度减小。毛细管液柱上升的高度与H_(2) S浓度在2.0~60μmol/L范围内呈线性关系,检出限为1.9μmol/L。本方法可用于红酒中H_(2) S的快速、简便测定。A portable sensor using the height of the liquid column in capillary as readout was designed for detection of hydrogen sulfide(H_(2)S).Cuprous ion(Cu^(+))could induce the combination of azide-modified magnetic beads(MBs-N_(3))and alkyne-modified platinum nanoparticles(Pt NPs-Alk)through click chemistry to form platinum nanoparticles connected to magnetic beads(MBs-Pt NPs).MBs-Pt NPs could be separated from the reaction mixture by magnetic force and transferred to a glass bottle filled with hydrogen peroxide(H_(2)O_(2))solution with a capillary on bottle cap.In the above-mentioned closed reaction device,H_(2)O_(2) was catalytically decomposed by Pt NPs and generated a large amount of oxygen,which caused the liquid column in the capillary to rise.In the presence of H_(2)S,the azide groups on the surface of MBs-N_(3) could be reduced to amino groups by H_(2)S,thereby reducing the click chemistry reaction sites between it and Pt NPs-Alk,ultimately leading to a reduction in the catalytic efficiency of H_(2)O_(2) decomposition and a reduction in the rising height of the capillary liquid column.The change of height of the liquid column in the capillary had a linear relationship with H_(2)S concentration in the range of 2.0 to 60μmol/L,and the limit of detection was 1.9μmol/L.The method was used for the rapid and simple determination of H_(2)S in red wine with satisfactory results.

关 键 词：点击化学 硫化氢 毛细管 液柱高度 便携式传感器 

分 类 号：TS262.6[轻工技术与工程—发酵工程] TP212[轻工技术与工程—食品科学与工程]