牙髓干细胞外泌体对口腔鳞状细胞癌CAL-27增殖、迁移的影响及其机制的研究  

Effects of exosomes derived from dental pulp stem cells on the proliferation and migration of oral squamous cell carcinoma CAL-27 and its mechanism

作　　者：岳杰 杨麒麟 孙翔宇[1] 孙誉铭 麻响 陈东[1] 静广平[1] UE Jie;YANG Qilin;SUN Xiangyu;SUN Yuming;MA Xiang;CHEN Dong;JING Guangping(The First Affiliated Hospital of Harbin Medical University,School of Stomatology,Harbin Medical University,Harbin 150001,China)

机构地区：[1]哈尔滨医科大学附属第一医院口腔颌面外科,哈尔滨150001

出　　处：《实用肿瘤学杂志》2022年第5期398-403,共6页Practical Oncology Journal

基　　金：哈尔滨医科大学附属第一医院科研创新基金(编号:2019M22)。

摘　　要：目的研究人牙髓干细胞来源的外泌体(human dental pulp stem cell-derived exosome,hDPSCs-exo)对口腔鳞状细胞癌细胞(CAL-27)增殖和迁移的影响。方法收集来哈尔滨医科大学附属第一医院治疗的16~28岁患者因阻生或正畸拔除的牙髓组织,通过超速离心法提取hDPSCs-exo,不同浓度hDPSCs-exo处理CAL-27细胞,通过MTT实验、Transwell实验和划痕实验检测CAL-27细胞增殖和迁移能力的变化;Western blot研究PI3K/Akt信号通路相关蛋白的表达。结果MTT实验结果显示,仅80μg/mL hDPSCs-exo组在第5d对CAL-27细胞的增殖有抑制作用(P<0.05),其余各浓度组均没有显著影响;Transwell和划痕实验结果显示20、60、80μg/mL组可以显著地促进CAL-27细胞的迁移(P<0.05);Western blot结果显示经hDPSCs-exo处理后,CAL-27细胞中PI3K/Akt信号通路相关蛋白PI3K、P-Akt的表达水平明显上调(P<0.05)。结论hDPSCs-exo高浓度时抑制CAL-27细胞的增殖,适宜浓度能显著促进CAL-27细胞的迁移能力,其作用机制可能与激活PI3K/Akt信号通路有关。Objective The objective of this study was to investigate the effects of human dental pulp stem cell derived exosomes(hDPSCs-exo)on the proliferation and migration of oral squamous carcinoma CAL-27 cells.Methods hDPSCs-exo was extracted by ultracentrifugation.CAL-27 cells were treated with different concentrations of hDPSCs-exo.The proliferation and migration of CAL-27 cells were detected by MTT assay,Transwell and scratch assays;Western Blot was used to detect the levels of PI3K/AKT signaling pathway related proteins.Results The results of MTT assay showed that only 80μg/mL of hDPSCs-exo had an inhibitory effect on the proliferation of CAL-27 cells on the 5th day(P<0.05),and the other concentration groups had no significant effect.The results of Transwell and scratch assays showed that 20,60,and 80μg/mL of hDPSCs-exo could significantly promote migration of CAL-27 cells(P<0.05).The results of Western blot showed that the levels of PI3K/AKT signaling pathway related proteins PI3K and P-AKT were significantly up-regulated in CAL-27 cells after hDPSCs-exo treatment(P<0.05).Conclusion High concentration of hDPSCs-exo can inhibit the proliferation of CAL-27 cells,and the appropriate concentration can significantly promote the migration of CAL-27 cells.Its mechanism of action may be related to the activation of the PI3K/AKT signaling pathway.

关 键 词：牙髓干细胞 外泌体 口腔鳞状细胞癌 PI3K/AKT 增殖 迁移 

分 类 号：R739.8[医药卫生—肿瘤]