游泳运动与饮食控制改善高脂饮食APOE^(-/-)小鼠下丘脑病变的机制  

Mechanisms by which swimming exercise and diet control improve hypothalamic lesions in APOE^(-/-) mice with high-fat diet

作　　者：丁琳琳 卢陶陶 魏伟 李永旭 林丽斌 林志诚 薛偕华 Ding Linlin;Lu Taotao;Wei Wei;Li Yongxu;Lin Libin;Lin Zhicheng;Xue Xiehua(College of Rehabilitation Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350108,Fujian Province,China;The Affiliated Rehabilitation Hospital,Fujian University of Traditional Chinese Medicine,Fuzhou 350003,Fujian Province,China;Fujian Provincial Key Laboratory of Cognitive Rehabilitation,Fuzhou 350003,Fujian Province,China)

机构地区：[1]福建中医药大学康复医学院,福建省福州市350108 [2]福建中医药大学附属康复医院,福建省福州市350003 [3]福建省认知功能康复重点实验室,福建省福州市350003

出　　处：《中国组织工程研究》2023年第20期3136-3142,共7页Chinese Journal of Tissue Engineering Research

基　　金：福建省自然科学基金(2020J01759),项目负责人:林志诚。

摘　　要：背景:游泳运动和饮食控制是目前公认的改善代谢异常的重要干预方式,下丘脑去乙酰化酶3、过氧化物酶体增殖物激活受体γ共激活因子1α蛋白是调控能量代谢的重要因子,但游泳运动和饮食控制对高脂饮食载脂蛋白E基因敲除(APOE^(-/-))小鼠下丘脑病变的影响及其与PGC-1α-SIRT3通路的相关性,目前尚不清楚。目的:探讨游泳运动与饮食控制调节高脂饮食APOE^(-/-)小鼠下丘脑病变的可能机制。方法:50只20周龄的SPF级雄性APOE^(-/-)小鼠随机分为对照组(正常饮食)、高脂饮食组、饮食控制组、游泳组、饮食控制+游泳组,每组10只。除对照组(普通饲料)外,其余各组均进行8周的高脂饲料(21%脂肪,0.15%胆固醇)饲养。饲养8周后,饮食控制组和饮食控制+游泳组改为普通饲料喂养,其余各组喂养方式不变;游泳组和饮食控制+游泳组进行无负重的游泳运动,共持续8周。每周的同一时间测量各组小鼠的体质量,MRI波谱检测下丘脑代谢物质N-乙酰天门冬氨酸、胆碱复合物、肌酸、肌醇的表达变化;电镜检测小鼠下丘脑的线粒体和髓鞘形态变化;蛋白质免疫印迹法检测下丘脑去乙酰化酶3、过氧化物酶体增殖物激活受体γ共激活因子1α蛋白的表达。结果与结论:①高脂饮食喂养后对照组体质量显著低于其余4组;干预8周后,游泳组、饮食控制组、饮食控制+游泳运动组体质量显著低于高脂饮食组(P<0.05),饮食控制+游泳运动的下降趋势最明显;②与对照组相比,高脂饮食组的N-乙酰天门冬氨酸、胆碱复合物显著减少,肌醇显著增加(P<0.05);与高脂饮食组相比,饮食控制组、游泳组和饮食控制+游泳组N-乙酰天门冬氨酸、胆碱复合物显著增加,肌醇显著减少(P<0.05),其中饮食控制+游泳组的作用更显著;③饮食控制组、游泳组、饮食控制+游泳组下丘脑的去乙酰化酶3和过氧化物酶体增殖物激活受体γ共激活因子BACKGROUND:Swimming exercise and diet control are currently recognized as important interventions to improve metabolic abnormalities.Hypothalamic sirtuin 3(SIRT3)and peroxisome proliferator-activated receptor-γcoactivator-1α(PGC-1α)are important factors regulating energy metabolism,but the correlation between the effects of swimming exercise and diet control on hypothalamic lesions in apolipoprotein E knockout(APOE^(-/-))mice with high-fat diet and the PGC-1α-SIRT3 pathway is still unclear.OBJECTIVE:To investigate the possible mechanism of swimming exercise and diet control in regulating hypothalamic lesions of APOE^(-/-)mice with high-fat diet.METHODS:Fifty 20-week-old APOE^(-/-)mice were randomly divided into normal diet group,high-fat diet group,diet control group,swimming exercise group and diet control+swimming exercise group,with 10 mice in each group.Except for the control group(normal diet),the other groups were fed with high-fat diet(21%fat,0.15%cholesterol)for 8 weeks.After 8 weeks of feeding,the diet control group and the diet control+swimming exercise group were normally fed,and there were no changes in the other groups.The swimming exercise group and the diet control+swimming exercise group were given no weight-bearing swimming for 8 weeks in total.Mice were weighed at the same time each week,and changes in hypothalamic metabolites,including N-acetyl aspartate(NAA),choline complex(Cho),creatine(Cr),and myo inositol(MI)were detected by magnetic resonance spectrum of the hypothalamus.Morphological changes of mitochondria and myelin sheath in the hypothalamus were detected by electron microscopy.SIRT3 and PGC-1αprotein expression was detected by western blot.RESULTS AND CONCLUSION:The body mass of the control group was significantly lower than that of the other four groups after high-fat diet feeding.After 8 weeks of intervention,the body mass of swimming exercise group,diet control group,diet control+swimming exercise group was significantly lower than that of high-fat diet group(P<0.05),and the d

关 键 词：游泳运动 饮食控制 高脂饮食 下丘脑 MRI波谱 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] G804.7[文化科学—运动人体科学]