模拟失重条件下小鼠视网膜坏死和凋亡的变化  

Retinal necrosis and apoptosis changes in mice under simulated microgravity

作　　者：杨振菲 李文炯[2] 张鹏[2] 查旭[3] 李霄 陈晓萍[2] 朱思泉 Zhen-Fei Yang;Wen-Jiong Li;Peng Zhang;Xu Zha;Xiao Li;Xiao-Ping Chen;Si-Quan Zhu(Department of Ophthalmology,Rehabilitation Hospital,National Research Center for Rehabilitation Technical Aids,Beijing 100176,China;State Key Laboratory of Space Medicine Fundamentals and Application,Astronaut Research and Training Center of China,Beijing 100094,China;Department of Ophthalmology,the Second Affiliated Hospital of Kunming Medical University,Kunming 650101,Yunnan Province,China;Department of Ophthalmology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan Province,China;Department of Ophthalmology,Beijing Anzhen Hospital,Capital Medical University,Beijing 100029,China)

机构地区：[1]国家康复辅具研究中心附属康复医院眼科,中国北京市100176 [2]中国航天员科研训练中心航天医学基础与应用国家重点实验室,中国北京市100094 [3]昆明医科大学第二附属医院眼科,中国云南省昆明市650101 [4]郑州大学第一附属医院眼科,中国河南省郑州市450052 [5]首都医科大学附属北京安贞医院眼科,中国北京市100029

出　　处：《国际眼科杂志》2022年第11期1765-1770,共6页International Eye Science

基　　金：National Defense Key Laboratory of Science and Technology Fund for Human Factors Engineering(No.6142222180103)。

摘　　要：目的:探索模拟失重环境与视网膜损伤的关系。方法:采用尾吊小鼠试验模拟失重,应用光镜和透射电镜观察视网膜组织形态学变化;应用免疫组织化学和Western blot检测与分析相关的分子变化。结果:光镜和透射电镜显示:与对照组相比,尾吊组小鼠视网膜神经节层和内核层细胞中均可见更多死亡细胞,相关通路中的TNF-α和caspase-3蛋白表达上调。结论:在模拟失重环境下小鼠视网膜会产生明显的病理损伤。AIM:To investigate whether the microgravity environment is related to retinal damage.METHODS:Hanging-tail mice tests were used to simulate weightlessness.Light microscopy,and transmission electron microscopic examinations of the retinal tissue structure were used to observe morphological changes.Immunohistochemistry and Western blot analyses were performed to detect the molecular changes associated with the observations.RESULT:Light microscopy and transmission electron microscopy demonstrated that more dead cells were detected in the ganglion layer and inner nuclear layer cells.TNF-αand caspase-3 protein expression in the retinas of simulated microgravity groups were up-regulated compared with the ground-based control group.CONCLUSION:The results demonstrated that simulated microgravity produced severe pathological damage in the retinas of mice.

关 键 词：模拟失重 视网膜 TNF-α CASPASE-3 小鼠 

分 类 号：R774.1[医药卫生—眼科]