三氯异氰尿酸对马铃薯连作障碍土壤微生物群落组成的影响  

Effects of Tarichloroisocyanuric Acid on Soil Microbial Community Composition Under Potato Continuous Cropping Obstacle

作　　者：周杨 石思雨 司友涛[1,2] 马红亮 高人[1,2] 尹云锋[1,2] ZHOU Yang;SHI Siyu;SI Youtao;MA Hongliang;GAO Ren;YIN Yunfeng(Institute of Geography,Fujian Normal University,Fuzhou 350007,China;Fujian Provincial Key Laboratory for Plant Eco-physiology,Fujian Normal University,Fuzhou 350007,China)

机构地区：[1]福建师范大学地理研究所,福州350007 [2]福建师范大学福建省植物生理生态重点实验室,福州350007

出　　处：《土壤》2022年第4期756-762,共7页Soils

基　　金：福建省自然科学基金项目(2021J01143);国家自然科学基金项目(31470628,31770659)资助。

摘　　要：采用盆栽试验,选择三氯异氰尿酸(TCCA)为土壤消毒剂,研究不同施用量TCCA对马铃薯连作障碍土壤微生物群落组成的影响。TCCA施用量设置分别为:0(CK),30 kg/hm^(2)(S_(30)),60 kg/hm^(2)(S_(60))和120 kg/hm^(2)(S_(120))。结果表明:与CK处理相比,S_(60)和S_(120)处理显著提高土壤pH(P<0.05),分别提高0.15个和0.26个单位;显著增加土壤可溶性有机碳含量(P<0.05),增幅分别为30.4%和83.5%;显著提高土壤铵态氮含量(P<0.05),分别提高3.3倍和9.7倍。同时,S_(60)和S_(120)处理显著影响了土壤微生物优势菌属的相对丰度(P<0.05)。与CK处理相比,在门水平上,S_(60)和S_(120)处理下土壤变形菌门(Proteobacteria)的增幅达55.3%和85.9%,子囊菌门(Ascomycota)的增幅达16.7%和35.1%;在属水平上,S_(60)和S_(120)处理下显著降低马铃薯致病菌链霉菌属(Streptomyces)的相对丰度(P<0.05),降幅达35.7%和57.1%。主坐标(PCoA)分析表明,S_(60)和S_(120)处理与CK处理的细菌和真菌的群落结构截然不同。除了TCCA直接杀菌的影响,冗余分析(RDA)显示,土壤pH和铵态氮含量亦是影响微生物群落组成变化的关键因子。In this study,a pot experiment was conducted to investigate the effects of different application rates of trichloroisocyanuric acid(TCCA)used as disinfectant on soil microbial community composition under potato continuous cropping obstacle.Four treatments of TCCA application rates were designed,including 0(CK),30 kg/hm^(2)(S_(30)),60 kg/hm^(2)(S_(60))and 120 kg/hm^(2)(S_(120)).Compared to CK,S_(60)and S_(120)significantly increase soil pH by 0.15 and 0.26 units(P<0.05),increase soil dissolved organic carbon(DOC)content by 30.4%and 83.5%(P<0.05),increase soil NH_(4)^(+)-N content by 3.3 and 9.7 times(P<0.05),respectively.S_(60)and S_(120)significantly shift the relative abundance of dominant microorganism at phylum and genus levels(P<0.05).Compared to CK,S_(60)and S_(120)increase the relative abundances of Proteobacteria and Ascomycota by 55.3%and 85.9%,16.7%and 35.1%at the phylum level,respectively;while decrease the relative abundance of potato pathogen Streptomyces by 35.7%and 57.1%(P<0.05)at the genus level,respectively.Principal coordinate analysis(PCoA)shows that S_(60)and S_(120)significantly change the community structures of bacteria and fungi compared to CK.In addition to the direct effects of TCCA,redundancy analysis(RDA)suggests that soil pH and NH_(4)^(+)-N content are also the key factors affecting microbial community composition.

关 键 词：三氯异氰尿酸 马铃薯 连作障碍 微生物群落组成 病原菌 

分 类 号：S532[农业科学—作物学]