蛇床子挥发油指标成分含量测定的研究  

Determination of the contents of index components in volatile oil of common cnidium fruit

作　　者：张迪[1] 曹远东 钟慧[1] 卢兴[1] 黄玲[1] 王艳宁[1] ZHANG Di;CAO Yuandong;ZHONG Hui;LU Xing;HUANG Ling;WANG Yanning(Department of Pharmacy,the First People’s Hospital of Nanning,Guangxi,Nanning 530022,China;Sichuan Jiuyuan Pharmaceutical Technology Co.,Ltd.,Sichuan,Mianyang 621000,China)

机构地区：[1]南宁市第一人民医院药学部,广西南宁530022 [2]四川九渊医药科技有限公司,四川绵阳621000

出　　处：《中国医药科学》2022年第19期80-84,共5页China Medicine And Pharmacy

基　　金：广西中医药适宜技术开发与推广项目(GZSY20-66);广西壮族自治区卫生和计划生育委员会科研课题(Z20171010);全国中药特色技术传承人才培训项目(国中医药人教函〔2018〕204号;国中医药人教函〔2019〕43号)。

摘　　要：目的建立一种有效、快速测定蛇床子挥发油中莰烯、β-蒎烯、乙酸龙脑酯3种活性成分含量的气相色谱法(GC)。方法提取不同产地(广西、安徽、河北正定、浙江、河北安国)的蛇床子挥发油,经气相色谱-质谱法(GC-MS)分析后选择含量较高的共有成分作为指标物质,通过气相色谱(GC)测定蛇床子挥发油中指标物质的具体含量。结果莰烯、β-蒎烯、乙酸龙脑酯的线性范围在64.60~1314.79μg/m(l r=0.9977)、13.27~267.78μg/ml(r=0.9980)、91.99~1479.73μg/ml(r=0.9998);经验证方法的精密度、稳定性、重复性良好,RSD值均小于5%,平均回收率分别为100.44%、97.11%、93.10%。各产地蛇床子挥发油中莰烯、β-蒎烯、乙酸龙脑酯含量范围分别为:39.86~48.49 mg/g、9.06~16.34 mg/g、39.00~59.28 mg/g。结论本实验所建立的方法简便、快速,增加了能反映蛇床子临床疗效的质量控制指标,为现有国家标准的完善提供依据。Objective To establish an effective gas chromatography(GC)for the rapid determination of camphene,β-pinene and bornyl acetate as three active components in the volatile oil of common cnidium fruit.Methods The volatile oils of common cnidium fruit from different origins(Guangxi,Anhui,Zhengding County in Hebei,Zhejiang,and Anguo City in Hebei)were extracted,the shared components with higher contents were selected as the index substances by gas chromatography-mass spectrometry(GC-MS),and the specific contents of these index substances were determined by GC.Results The linear ranges of camphene,β-pinene and bornyl acetate were 64.60-1314.79μg/ml(r=0.9977),13.27-267.78μg/ml(r=0.9980),and 91.99-1479.73μg/ml(r=0.9998),respectively.The precision,stability and reproducibility of the method were verified to be good,with the relative standard deviation(RSD)values less than 5%,and the average recoveries were 100.44%,97.11%and 93.10%,respectively.The contents of camphene,β-pinene and bornyl acetate in the volatile oil of common cnidium fruit from various origins were ranged from 39.86-48.49 mg/g,9.06-16.34 mg/g,and 39.00-59.28 mg/g,respectively.Conclusion The method established in this experiment is simple and enables rapid determination of content.It also increases the quality control indexes that can reflect the clinical efficacy of common cnidium fruit,and provides a basis for the improvement of the existing national standards.

关 键 词：蛇床子 挥发油 气相色谱-质谱 气相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]