都匀毛尖本地种茶树响应假眼小绿叶蝉侵害的代谢组学研究  

Metabolomics study on the response of Duyun Maojian native tea plant to the infestation of Empoasca vitis Gothe

作　　者：王芬 木仁 李国栋 喻佩 李静 崔宝禄 吕立堂 WANG Fen;MU Ren;LI Guodong;YU Pei;LI Jing;CUI Baolu;LYU Litang(The Department of Life Science and Agriculture,Qiannan Normal University for Nationalities,Duyun,Guizhou 558000,China;College of Tea Science,Guizhou University,Guiyang,Guizhou 550025,China)

机构地区：[1]黔南民族师范学院生物科学与农学院,贵州都匀558000 [2]贵州大学茶学院,贵州贵阳550025

出　　处：《湖南农业大学学报（自然科学版）》2022年第5期543-549,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(32260080、31900486);贵州省教育厅植物学重点支持学科项目(黔学位合字ZDXK[2016]23号);贵州省科技计划项目(黔科合基础[2019]1298);贵州省教育厅项目([2015]68、QNYSKYTD2018006);黔南民族师范学院科研创新团队项目(Qnsyk201605、QNYSKYTD2018011);黔南民族师范学院项目(2019xjg0303、2018xjg0520、QNSY2018ZJ006、QNYSXXK2018005)。

摘　　要：以被假眼小绿叶蝉侵染0、12、24 h的都匀毛尖本地种茶树叶片为试验材料,采用超高效液相色谱–串联四级杆飞行时间质谱技术对试验材料的代谢产物进行定性和定量分析,正离子模式下鉴定出1094个代谢物。采用主成分分析、正交偏最小二乘法判别分析,根据差异倍数筛选出0 h与12 h处理有20个差异代谢物,其中6个差异代谢物的含量上调,14个下调;0 h与24 h处理之间有41个差异代谢物,其中26个上调,15个下调;12 h与24 h处理有59个差异代谢物,其中45个上调,14个下调。0 h与12 h处理有2个差异代谢物注释到KEGG数据库中,其中芥子酸注释到苯丙素生物合成途径中,与木质素形成有关;0 h与24 h处理有6个差异代谢物注释到KEGG数据库中,其中6,9,12,15十八碳四烯酸乙酯注释到亚麻酸代谢通路,没食子儿茶素注释到类黄酮生物合成,苯乙醛注释到苯丙氨酸代谢通路,推测苯乙醛会对周围的假眼小绿叶蝉造成其行为或生理的影响,而苯丙氨酸经苯丙氨酸解氨酶等酶催化合成木质素和类黄酮等;12 h与24 h处理有9个差异代谢物注释到9条通路中,马钱苷酸注释到单萜生物合成通路中,相比侵染12 h处理,24 h处理的马钱苷酸含量上调,表明茶树被假眼小绿叶蝉为害后可能通过释放萜类物质来抵御害虫。以上结果表明,木质素、萜类物质和黄酮类化合物在茶树抵御小绿叶蝉的侵害具有重要作用。To investigate how tea response to Empoasca vitis infection,we used ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS)to measure the profiles of 1094 metabolites in tea leaves at 0 h,12 h and 24 h after infection.With principal component analysis(PCA),orthogonal partial least squares discrimination analysis(OPLS-DA),and fold change(FC),6 metabolites were upregulated and 14 downregulated at 12 h comparing to 0 h,26 metabolites were upregulated and 15 were downregulated at 24 h compared to 0 h,and 45 metabolites were upregulated and 14 were downregulated at 24 h compared to 12 h.Two differential metabolites in phenylpropanoid biosynthesis pathway with sinapic acid related to lignin formation at 0 h and 12 h were annotated to KEGG database.Six differential metabolites whose phenylacetaldehyde and gallocatechin were annotated to phenylalanine metabolic pathway and biosynthesis of flavonoids,respectively at 0 h and 24 h were also annotated to KEGG database.It was speculated that the fragrance of phenylacetaldehyde would affect the behavior or physiology of Empoasca vitis Gothe.There were 9 differential metabolites at 12 h and 24 h annotated to 9 metabolic pathways,and loganic acid annotated to monoterpenoid biosynthesis pathway.Compared with 12 h,the increase of loganic acidin in 24 h indicated that tea plants might release terpenoids to resist the Empoasca vitis Gothe.The results indicated that lignin,terpenoids,and flavonoids played important roles in the response to Empoasca vitis infection.

关 键 词：茶叶 假眼小绿叶蝉 代谢组学 超高效液相色谱–串联四级杆飞行时间质谱 差异代谢物 

分 类 号：S571.101[农业科学—茶叶生产加工] S435.711[农业科学—作物学]