鼻咽癌同期放化疗抵抗细胞株与母细胞株lncRNA的差异表达谱分析  

Study on Differential Expression Profile of lncRNA between Concurrent Chemoradioresistant Nasopharyngeal Carcinoma Cell Lines and Parental Cell Lines

作　　者：刘秋燕 熊伟 李程 Qiuyan LIU;Wei XIONG;Cheng LI(Dept.of Radiation Therapy,Yunnan Cancer Hospital,The 3rd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650118;Dept.of Oncology,Affiliated Hospital of Panzhihua University,Panzhihua Sichuan 617000,China)

机构地区：[1]云南省肿瘤医院/昆明医科大学第三附属医院放射治疗中心,云南昆明650118 [2]攀枝花学院附属医院肿瘤科,四川攀枝花617000

出　　处：《昆明医科大学学报》2022年第11期77-84,共8页Journal of Kunming Medical University

基　　金：云南省卫生科技计划基金资助项目(2018NS0066);云南省教育厅科学研究基金资助项目(2018JS222)。

摘　　要：目的分析鼻咽癌同期放化疗抵抗细胞株与亲本细胞株间差异表达的长链非编码RNA(long noncoding RNA,lncRNA),探索lncRNA在鼻咽癌同期放化疗抵抗中可能发挥的作用。方法体外构建鼻咽癌CEN1和CNE2细胞同期放化疗抵抗模型,诱导鼻咽癌细胞同期放化疗抵抗细胞株CEN1CRR和CNE2CRR,应用高通量测序筛选2组细胞株间差异表达的lncRNAs,对差异表达的lncRNAs的靶基因进行GO和KEGG分析。结果以Log2FC>1或<-1,FDR<0.05为差异表达标准,和CNE1相比,CNE1CRR差异表达的lncRNAs2746个(358个上调,2063个下调);和CNE2相比,CNE2CRR差异表达的lncRNAs3475个(265个上调,520个下调);此外,387个lncRNAs在CNE1CRR和CNE2CRR中表达同时下调,49个lncRNAs在CNE1CRR和CNE2CRR中同时上调。在GO分析中发现,2组细胞中差异表达lncRNAs的靶基因在生物过程(biological process,BP)、分子功能(molecular function,MF)、细胞成分(cellular component,CC)等方面均有参与。在KEGG分析结果中发现,CNE1组细胞中,差异lncRNAs的靶基因主要富集的信号通路有细胞凋亡、病毒致癌、代谢途径等。CNE2组细胞中,差异lncRNAs的靶基因主要富集的信号通路有代谢途径(硫辛酸、磷酸戊糖、花生四烯酸、醚脂质)、T细胞受体信号通路、核苷酸切除修复等(P<0.05)。结论同期放化疗抵抗细胞株与亲本细胞株的lncRNA表达谱存在明显差异,这些差异表达的lncRNA可能参与了鼻咽癌的同期放化疗抵抗,并为其机制研究奠定基础。Objective To analyze the long non coding RNA(lncRNA) differentially expressed between nasopharyngeal carcinoma cell lines resistant to concurrent radiotherapy and chemotherapy and their parent cell lines,and explore the possible role of lncRNA in nasopharyngeal carcinoma resistance to concurrent radiotherapy and chemotherapy. Methods The concurrent chemoradiotherapy resistance model of nasopharyngeal carcinoma CEN1and CNE2 cells was constructed in vitro,and the concurrent chemoradiotherapy resistance cell lines CEN1CRR and CNE2CRR of nasopharyngeal carcinoma cells were induced. The differentially expressed lncRNAs between the two groups of cell lines were screened by high-throughput sequencing, and the target genes of the differentially expressed lncRNAs were analyzed by GO and KEGG.Results With Log2FC>1 or<-1 and FDR<0.05 as the differential expression criteria,2 746 lncRNAs (358 up-regulated and 2 063 down-regulated) were differentially expressed in CNE1 CRR compared with CNE1.Compared with CNE2,CNE2CRR differentially expressed 3 475lncRNAs (265 up-regulated,520 down-regulated).In addition,387 lncRNAs were downregulated in CNE1CRR and CNE2CRR,and 49 lncRNAs were upregulated in CNE1CRR and CNE2CRR.GO analysis showed that the target genes differentially expressing lncRNAs in the two groups of cells were involved in biological process (BP),molecular function (MF),and cell component (CC).In the KEGG analysis results,it was found that in CNE1group cells,the main signal pathways for the enrichment of target genes of differential lncRNAs were apoptosis,viral carcinogenesis,metabolic pathways,etc.In CNE2 group,the target genes of differential lncRNAs were mainly enriched through metabolic pathways (lipoic acid,pentose phosphate,arachidonic acid,ether lipid),T cell receptor signaling pathways,nucleotide excision repair,etc.(P<0.05).Conclusions There are significant differences in lncRNA expression profiles between concurrent chemoradiotherapy-resistant cell lines and parental cell lines.These differentially expressed ln

关 键 词：长链非编码RNA(lncRNA) 表达谱 鼻咽癌细胞 同期放化疗抵抗 高通量测序 

分 类 号：R73-3[医药卫生—肿瘤]