人肌层浸润性膀胱癌组织及血清中circRHOT1表达及临床意义  

Expression and clinical significance of circRHOT1 in human muscle invasive bladder cancer tissues and serum

作　　者：张玉瑞[1] 朱海鹏[1] 王栋[1] 史校学 康郑军[1] ZHANG Yurui;ZHU Haipeng;WANG Dong;SHI Xuexue;KANG Zhengjun(Department of Urology,the Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州郑州大学第五附属医院泌尿外科,郑州450052

出　　处：《临床肿瘤学杂志》2022年第10期903-909,共7页Chinese Clinical Oncology

基　　金：河南省高等学校重点科研项目资助计划(21A320037);河南省医学科技攻关计划资助项目(LHGJ20220564)。

摘　　要：目的 探讨肌层浸润性膀胱癌(MIBC)患者组织和血清中circRHOT1表达及临床意义。方法 选取2011年3月至2017年4月在我院泌尿外科接受根治性膀胱切除术的91例MIBC患者作为研究对象,另选取同期在本院体检健康且无膀胱癌病史的志愿者80例作为健康对照组。采用实时荧光定量PCR(qPCR)检测组织和血清circRHOT1表达的关系。采用受试者特征工作曲线(ROC)评价血清circRHOT1表达诊断MIBC的效能。Spearman相关性分析分析组织和血清circRHOT1表达。Kaplan-meier法绘制总生存期(OS)、疾病特异性生存期(DSS)、无复发生存期(RFS)曲线。Cox风险比例回归模型分析影响MIBC预后的因素。结果 MIBC患者血清circRHOT1表达水平显著高于健康对照组[1.49(1.07,2.10) vs.1.00(0.82,1.26),P<0.001]。血清circRHOT1水平诊断MIBC的ROC曲线下面积为0.774(95%CI:0.705~0.843)。MIBC组织circRHOT1表达水平显著高于癌旁组织[1.83(1.45,2.62) vs.1.09(0.85,1.33),P<0.001]。Spearman相关性分析结果显示,血清circRHOT1水平与组织circRHOT1表达呈正相关(r=0.388,P<0.001)。MIBC患者血清和组织circRHOT1表达仅与Ki-67有关(P<0.05)。组织circRHOT1低表达和高表达组患者中位OS分别为2.52年、1.90年(P=0.001),中位DSS为2.52年、1.90年(P=0.004),中位RFS为1.79年、1.16年(P=0.001)。血清circRHOT1低水平和高水平组患者中位OS分别为2.09年、1.98年(P=0.006),中位DSS为2.09年、1.98年(P=0.019),中位RFS为1.61年、1.16年(P=0.003)。经单因素和多因素Cox风险比例回归分析,血清circRHOT1表达影响MIBC患者术后OS、DSS和RFS的独立预后因素(P<0.05)。结论circRHOT1在MIBC组织和血清中高表达,且其高表达与复发及死亡高风险相关。血清circRHOT1水平可作为MIBC诊断生物标志物。Objective To investigate the expression and clinical significance of circRHOT1 in tissue and serum of patients with muscle-invasive bladder cancer(MIBC).Methods Ninety-one patients with MIBC who underwent radical cystectomy in Urology Department of our hospital from March 2011 to April 2017 were selected as the research objects,and 80 healthy volunteers without bladder cancer history in our hospital during the same period were selected as healthy control group.The expression of circRHOT1 in tissues and serum was detected by quantitative real-time PCR(qPCR).The receiver characteristic operating curve(ROC)was used to evaluate the diagnostic efficiency of serum circRHOT1 expression in MIBC.Spearman correlation analysis was used to analyze the expression of circRHOT1 in tissues and serum.The overall survival(OS),disease-specific survival(DSS)and relapse-free survival(RFS)curves were drawn by Kaplan-Meier method.Cox proportional hazard model was used to analyze the prognostic factors of MIBC.Results The expression level of circRHOT1 in serum of MIBC patients was significantly higher than that of healthy controls[1.49(1.07,2.10)vs.1.00(0.82,1.26),P<0.001].The area under ROC curve of circRHOT1 level in diagnosing MIBC was 0.774(95%CI:0.705-0.843).The expression level of circRHOT1 in MIBC tissues was significantly higher than that in adjacent tissues[1.83(1.45,2.62)vs.1.09(0.85,1.33),P<0.001].Spearman rank correlation analysis showed that serum circRHOT1 level was positively correlated with tissue circRHOT1 expression(r=0.388,P<0.001).The expression of circRHOT1 in serum and tissues of MIBC patients was only related to Ki-67(P<0.05).The median OS was 2.52 years and 1.90 years(P=0.001),the median DSS was 2.52 years and 1.90 years(P=0.004),and the median RFS was 1.79 years and 1.16 years(P=0.001).The median OS was 2.09 years and 1.98 years(P=0.006),the median DSS was 2.09 years and 1.98 years(P=0.019),and the median RFS was 1.61 years and 1.16 years(P=0.003).Univariate and multivariate Cox proportional hazard regression an

关 键 词：肌层浸润性膀胱癌 circRHOT1 预后 临床意义 

分 类 号：R737.14[医药卫生—肿瘤]