千金藤素通过PI3K-AKT信号通路抑制前列腺癌细胞DU145增殖、侵袭和迁移  

Cepharanthine inhibits proliferation,invasion and migration of prostate cancer cell DU145 through PI3K-AKT signaling pathway

作　　者：梁泽旭 杨万山[2] 董秀哲(指导)[1] LIANG Zexu;YANG Wanshan;DONG Xiuzhe(Affiliated Hospital of Yanbian University,Yanji 133000,China)

机构地区：[1]延边大学附属医院,延吉133000 [2]延边大学医学院病理学教研室,延吉133002

出　　处：《中国免疫学杂志》2022年第16期1963-1965,1971,共4页Chinese Journal of Immunology

基　　金：吉林省教育厅“十三五”科学技术项目(JJKH20200524KJ)。

摘　　要：目的:探讨千金藤素(CEP)对前列腺癌细胞株DU145增殖、侵袭和迁移的影响及作用机制。方法:CCK-8测定药物浓度对细胞增殖的影响,细胞免疫荧光测定上皮-间充质转化(EMT)相关蛋白荧光强度,迁移实验及划痕实验测定细胞转移能力,Western blot检测EMT相关蛋白及PI3K-AKT信号通路蛋白表达。结果:CEP≥10μmol/L时,可抑制DU145细胞增殖(P<0.05),Vimentin和Snail表达下降,E-cadherin表达增加(P<0.05),Vimentin荧光强度减弱,E-cadherin荧光强度增强(P<0.05),细胞侵袭迁移能力均被抑制(P<0.05),p-PI3K及p-AKT蛋白表达降低(P<0.05)。结论:CEP可能通过PI3K-AKT信号通路抑制前列腺癌细胞DU145增殖、侵袭和迁移。Objective:To investigate effects and mechanism of cepharanthine(CEP)on proliferation,invasion and migration of prostate cancer cell DU145.Methods:Effect of drug concentration on cell proliferation was determined by CCK-8,fluorescence intensities of epithelial-mesenchymal transition(EMT)-related proteins were determined by cell immunofluorescence,and cell metastasis ability was determined by Transwell assay and wound healing assay. Expressions of EMT-related proteins and PI3K-AKT signaling pathway proteins were detected by Western blot.Results:CEP≥10 μmol/L,DU145 cell proliferation was inhibited(P<0.05),Vimentin and Snail expressions were decreased,E-cadherin expression was increased(P<0.05),Vimentin fluorescence intensity was decreased and E-cadherin fluorescence intensity was enhanced(P<0.05),invasion and migration ability of cells were inhibited(P<0.05),p-PI3K and p-AKT protein expressions were reduced(P<0.05).Conclusion:CEP may inhibit proliferation,invasion and migration of prostate cancer cells DU145 through PI3K-AKT signaling pathway.

关 键 词：千金藤素 增殖 上皮-间充质转化 PI3K-AKT 侵袭 

分 类 号：R737.25[医药卫生—肿瘤]