啤酒基荧光碳点的绿色合成及其在Fe^(3+)检测和细胞成像中的应用  

Green Synthesis of Beer-based Fluorescent Carbon Dots and Their Application in Fe^(3+) Detection and Cell Imaging

作　　者：胡金双 杨熙 刘舒芹 杨升宏 HU Jin-shuang;YANG Xi;LIU Shu-qin;YANG Sheng-hong(Shandong Provincial Key Laboratory of Molecular Engineering,School of Chemistry and Chemical Engineering,Qilu University of Technology(Shandong Academy of Science),Jinan 250353,China;Institute of Analysis,Guangdong Academy of Science(China National Analytical Center Guangzhou),Guangzhou 510070,China)

机构地区：[1]齐鲁工业大学(山东省科学院)化学与化工学院山东省分子工程重点实验室,山东济南250353 [2]广东省科学院测试分析研究所(中国广州分析测试中心),广东广州510070

出　　处：《分析测试学报》2022年第11期1644-1651,共8页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(22006143,21806189)。

摘　　要：以啤酒为碳源,通过水热法合成了一系列啤酒基荧光碳点,其中以齐鲁工业大学自酿啤酒合成的碳点相比其他品牌啤酒合成的碳点具有更好的发光效率。该碳点表现出发射波长随激发波长变化而变化的性质,最大发射波长为475 nm,呈现蓝色荧光。利用透射电子显微镜、X射线衍射技术、傅里叶变换红外光谱、紫外-可见吸收光谱和荧光光谱等对碳点结构及其光学特性进行了表征。基于Fe^(3+)对碳点的特异性荧光猝灭,建立了灵敏的Fe^(3+)检测方法,线性范围为0.3~45μmol/L,检出限达91 nmol/L,并成功用于实际水样中Fe^(3+)浓度的分析。所合成的碳点具有良好的生物相容性和细胞膜穿透性,可用于细胞内Fe^(3+)的可视化成像分析和多色细胞成像。Ferric ion with an abnormal concentration level can cause obvious body dysfunction,and even lead to Parkinson's disease,Alzheimer's disease and other serious diseases.Therefore,it is very important to develop an effective analytical method for the detection of Fe^(3+)in environmental sam⁃ples and the dynamic monitoring of Fe^(3+)in cells for the maintenance of normal physiological function of human body.In this study,a series of beer-based fluorescent carbon dots(CDs)were synthesized by hydrothermal method using beers as carbon sources.The CDs synthesized with the self-brewed beer of Qilu university of technology had better luminous efficiency than those synthesized with other brands.The structure and optical properties of CDs were characterized by transmission electron mi⁃croscopy(TEM),X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR),UVVis absorption spectroscopy(UV-Vis)and fluorescence spectroscopy.The results showed that the CDs were spherical monodispersed nanoparticles with a relatively narrow size distribution and an aver⁃age diameter of 5.6 nm,which had an amorphous carbon structure and contained a large number of active functional groups such as C—N/C—O and C==O on the surface.The CDs were determined to be composed of carbon,nitrogen and oxygen by X-ray photoelectricity spectroscopy.The CDs shows the property that the emission wavelength changes with the excitation wavelength,and the maximum emission wavelength is 475 nm,showing blue fluorescence.At the excitation wavelength of 400 nm,it has stable fluorescence in the pH range of 3.0-12.0.The effects of UV irradiation time and salt concentration on fluorescence intensity were investigated.The fluorescence intensity decreased by less than 6%even after 6 h UV irradiation.When the salt concentration was up to 0.5 mol/L,the fluorescence intensity did not change significantly.Therefore,the CDs has good optical stability.Based on the specific fluorescence quenching of CDs by Fe^(3+),a sensitive Fe^(3+)detection method was establish

关 键 词：碳点 水热合成 啤酒 Fe^(3+)检测 细胞成像 

分 类 号：O657.3[理学—分析化学] R97[理学—化学]