Functional structure analysis and genome‑wide identification of CNX gene family in cotton  

作　　者：XU Nan ZHANG Hong ZHANG Yuexin FAN Yapeng WANG Jing MALIK Waqar Afzal RUI Cun HAN Mingge LU Xuke CHEN Xiugui WANG Junjuan WANG Delong WANG Shuai CHEN Chao GUO Lixue ZHAO Lanjie YE Wuwei 

机构地区：[1]Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base,State Key Laboratory of Cotton Biology/School of Agricultural Sciences,Zhengzhou University,Anyang 455000,Henan,China

出　　处：《Journal of Cotton Research》2022年第3期285-301,共17页棉花研究（英文）

基　　金：supported by the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences,and Supported by China Agriculture Research System of MOF and MARA.

摘　　要：Background:Under abiotic stress conditions,cotton growth is inhibited and yield losses are severe.Identification of calnexin family members and function analysis under abiotic stress laid the foundation for the screening of stressrelated candidate genes.Results:A total of 60 CNX family members have been identified in Gossypium hirsutum,G.barbadense,G.arboreum,and G.raimondii,and they were divided into two categories:CNX and CRT genes.Through the construction of a phylogenetic tree,they were subdivided into three classes.Further analysis of chromosome localization,conserved promoters,gene structure and selection under pressure showed that the family members were highly conserved in the evolution process.Analysis of cis-acting elements in the promoter regions showed that CNX family genes contain regulatory elements for growth and development,anaerobic,drought,defense and stress response,and plant hormones.Using RNA-seq data to study the expression pattern of GhCNX genes under cold,hot,salt stress and Polyethylene glycol,it was observed that the gene expression levels changed by different degrees under different stress conditions,indicating that GhCNX members were involved in the regulation of multiple biological stresses.Conclusion:This study provides an insight into the members of cotton CNX genes.The results of this study suggested that CNX family members play a role in defense against adversity and provide a foundation for the discovery of stress-related genes.

关 键 词：CALNEXIN GhCNX Conserved motifs Selection pressure COLLINEARITY Differential expression 

分 类 号：S562[农业科学—作物学]