紫地榆防龋牙膏对牛切牙早期釉质龋的生物矿化作用  

Biomineralization Effect of Early Enamel Caries in Bovine Incisors Treated with Geranium strictipes Anti-Caries Toothpaste

作　　者：徐磊 李秋艳 王丽梅 蓝海 Xu Lei;Li Qiuyan;Wang Limei;Lan Hai(Colleg of Pharmacy,Dali University,Dali,Yunnan 671000,China;Department of Stomatology,Dali Bai Autonomous Prefecture People's Hospital,Dali,Yunnan 671000,China)

机构地区：[1]大理大学药学院,云南大理671000 [2]大理白族自治州人民医院口腔科,云南大理671000

出　　处：《大理大学学报》2022年第10期11-14,共4页Journal of Dali University

基　　金：国家自然科学基金项目(81260512)。

摘　　要：目的:研究紫地榆防龋牙膏对牛切牙釉质龋的生物矿化作用。方法:将紫地榆提取物乙酸乙酯部位和正丁醇部位加入牙膏基质制得紫地榆药物牙膏,测定其对致龋菌的最低抑菌浓度(MIC)。将MIC值作为实验浓度,测脱矿前后以及再矿化后的表面显微硬度值,计算釉质表面显微硬度恢复率;用扫描电镜观察牛切牙表面形貌。结果:乙酸乙酯部位牙膏组和正丁醇部位牙膏组的表面显微硬度恢复率分别为(97±45.61)%、(87±40.45)%,与去离子水组比较差异均有统计学意义(P<0.05)。扫描电镜观察,经紫地榆防龋牙膏稀释液处理后的牙釉质,结构平整光滑致密,表面无明显腔隙,存在少量的沉淀物。结论:紫地榆防龋牙膏能抑制口腔主要致龋菌的生长,对牛切牙釉质龋有明显的生物再矿化作用。Objective:To study the biomineralization effect of Geranium strictipes anti-caries toothpaste on the enamel caries of bovine incisors. Methods:The ethyl acetate part and n-butanol part of G. strictipes extract were added into the toothpaste matrix to prepare the medicinal toothpaste of G. strictipes. The minimum inhibitory concentration(MIC) of the toothpaste against cariogenic bacteria was determined. The MIC value was used as the experimental concentration to measure the microhardness values before and after demineralization and after remineralization,and the recovery rate of microhardness of enamel surface was calculated. Scanning electron microscope was used to observe the surface morphology of bovine incisors. Results:The recovery rate of surface microhardness of the ethyl acetate fraction group and the n-butanol fraction group were(97 ±45.61) % and(87 ±40.45) %,respectively,which were significantly different from those of the deionized water group(P <0.05). Scanning electron microscope observation showed that the enamel treated by the diluted solution of G. strictipes anti-caries toothpaste had a smooth and compact structure,with no obvious cavity on the surface,but there was a small amount of sediment. Conclusion:The G. strictipes anti-caries toothpaste can inhibit the growth of main oral cariogenic bacteria and has obvious biomineralization effect on the enamel caries of bovine incisors.

关 键 词：紫地榆 牙膏 致龋菌 釉质龋 再矿化 

分 类 号：R781.1[医药卫生—口腔医学]