A molecular probe carrying anti-tropomyosin 4 for early diagnosis of cerebral ischemia/reperfusion injury  

作　　者：Teng-Fei Yu Kun Wang Lu Yin Wen-Zhe Li Chuan-Ping Li Wei Zhang Jie Tian Wen He 

机构地区：[1]Department of Ultrasound,Beijing Tiantan Hospital,Capital Medical University,Beijing,China [2]CAS Key Laboratory of Molecular Imaging,Institute of Automation,Chinese Academy of Sciences,Beijing,China [3]State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University,Beijing,China [4]Anhui Province Key Laboratory of Functional Coordinated Complexes for Materials Chemistry and Application,School of Chemical and Environmental Engineering,Anhui Polytechnic University,Wuhu,Anhui Province,China [5]Beijing Advanced Innovation Center for Big Data-Based Precision Medicine,School of Engineering Medicine,Beihang University,Beijing,China

出　　处：《Neural Regeneration Research》2023年第6期1321-1324,共4页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81730050(to WH).

摘　　要：In vivo imaging of cerebral ischemia/reperfusion injury remains an important challenge.We injected porous Ag/Au@SiO_(2) bimetallic hollow nanoshells carrying anti-tropomyosin 4 as a molecular probe into mice with cerebral ischemia/reperfusion injury and observed microvascular changes in the brain using photoacoustic imaging with ultrasonography.At each measured time point,the total photoacoustic signal was significantly higher on the affected side than on the healthy side.Twelve hours after reperfusion,cerebral perfusion on the affected side increased,cerebrovascular injury worsened,and anti-tropomyosin 4 expression increased.Twenty-four hours after reperfusion and later,perfusion on the affected side declined slowly and stabilized after 1 week;brain injury was also alleviated.Histopathological and immunohistochemical examinations confirmed the brain injury tissue changes.The nanoshell molecular probe carrying anti-tropomyosin 4 has potential for use in early diagnosis of cerebral ischemia/reperfusion injury and evaluating its progression.

关 键 词：cerebral ischemia/reperfusion injury diagnosis dynamic monitoring ischemic stroke middle cerebral artery occlusion molecular probe NANOSHELLS photoacoustic imaging tropomyosin 4 ULTRASOUND 

分 类 号：R743.31[医药卫生—神经病学与精神病学]