程序性低温冷冻对自体移植牙牙周愈合的影响  

Effect of cryopreservation on periodontal healing of autotransplantation teeth

作　　者：骆敬 隆元锶 易守银 冉娟 田鲲 LUO Jing;LONG Yuansi;YI Shouyin;RAN Juan;TIAN Kun(School of Stomatology,Southwest Medical University,Luzhou 646000,Sichuan,China;School of Stomatology,Zunyi Medical University,Zunyi 563003,Guizhou,China;Department of Stomatology,Sichuan Academy of Medical Sciences,Sichuan Provincial People's Hospital,Chengdu 610072,China)

机构地区：[1]西南医科大学口腔医学院,四川泸州646000 [2]遵义医科大学口腔医学院,贵州遵义563003 [3]四川省医学科学院·四川省人民医院口腔科,四川成都610072

出　　处：《西部医学》2022年第11期1576-1580,1587,共6页Medical Journal of West China

基　　金：四川省青年科技创新研究团队科研资助项目(2021JDTD0005)。

摘　　要：目的探讨通过程序性降温法低温冷冻处理移植供牙牙体,行自体异位移植术后评价牙周组织的生长、愈合情况。方法选取1年龄比格犬1只,体重9 kg,拔除左侧下颌第三侧切牙置于10%二甲亚砜保存液80℃冻存2周,程序性复苏解冻后移植于右下颌同名牙位作为冷冻牙,左侧下颌第二侧切牙即刻移植于右下颌同名牙位作为对照牙,喂养3月,比较两牙的临床表现、X射线检查、micro CT及组织学染色评估牙周愈合情况。结果术后1月冷冻移植牙和即刻移植牙牙龈缘轻微红肿、BOP(探诊出血)+,松Ⅰ°,X射线片示冷冻牙根尖周低密度透射影像,无牙根内外吸收影像。术后3月两牙牙龈红肿,BOP+、松Ⅰ-Ⅱ°,探及轻度牙周袋,X射线片示两牙牙根表面出现不同程度凹陷性吸收,冷冻牙近中根尖处牙骨质可见明显吸收,无牙根内吸收影像。Micro CT影像显示即刻移植牙术后3月牙根表面略微粗糙,局部有微小浅表性吸收,根尖区无透射影;冷冻移植牙术后3月出现较大范围吸收,波及牙本质,并伴有根尖部牙槽骨吸收。组织学HE染色切片示冷冻牙牙根表面吸收,牙颈部结合上皮再附着松弛,而对照牙牙根吸收较轻,结合上皮再附着更致密。结论选用合适冷冻保护剂和冷冻方法的情况下冷冻移植牙牙周预后是可以预期的,但是仍然需要进行更多的研究以预防牙根吸收。Objective To evaluate the growth and healing of periodontal tissue after autologous heterotopic transplantation.Methods A 1-year-old beagle dog was used in the experiment.The left mandibular third incisor was extracted and frozen in 10%dimethyl sulfoxide preservation solution 80℃for 2 weeks.After programmed resuscitation and thawing,it was transplanted into the same tooth position of the right mandibular.The left mandibular second incisor was immediately transplanted into the same tooth position of the right mandibular as the control.It was fed for 3 months.The periodontal healing was evaluated by clinical observation,X-ray examination,micro CT and histological staining.Results One month after operation,the gingival margin of frozen transplanted teeth and immediate transplanted teeth was slightly red and swollen BOP(probing bleeding)+looseningⅠ°.X-ray film showed low-density transmission image around the frozen tooth root tip,without internal and external root absorption image.3 months after operation,the gums of both teeth were red and swollen,BOP+,looseningⅠ-Ⅱ°,probing and mild periodontal pocket.X-ray film showed concave absorption in varying degrees on the surface of both teeth root,obvious absorption of cementum near the middle root tip of frozen teeth,and no internal root absorption image.Micro CT The images showed that 3 months after immediate tooth transplantation,the root surface was slightly rough,there was slight superficial absorption in some parts,and there was no penetrating shadow in the apical area.A large range of absorption occurred 3 months after cryotransplantation,affecting the dentin,accompanied by apical alveolar bone absorption.Histological HE staining sections showed that the root surface of frozen teeth was absorbed,and the reattachment of cervical junction epithelium was loose.The root resorption of the control teeth was lighter,and the reattachment of the binding epithelium was more dense.Conclusion With the selection of appropriate cryoprotectants and freezing methods,the pe

关 键 词：比格犬 自体牙移植 低温冷冻 牙周愈合 

分 类 号：R782.12[医药卫生—口腔医学]