FGF3在舌鳞癌中的表达及对舌鳞癌细胞增殖及迁移的影响  

Expression of FGF3 in tongue squamous cell carcinoma and its effect on proliferation and migration of tongue squamous cell carcinoma cells

作　　者：张洋[1] 魏向华 刘攀[1] 敖登高娃 宿伟鹏[1] 赵化荣[1] ZHANG Yang;WEI Xianghua;LIU Pan;Aodenggaowa;SU Weipeng;ZHAO Huarong(Department of Oncology,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆医科大学第一附属医院肿瘤科,新疆乌鲁木齐830000

出　　处：《西部医学》2022年第11期1595-1599,共5页Medical Journal of West China

基　　金：新疆维吾尔自治区自然科学基金(2018D01C213)。

摘　　要：目的探讨成纤维生长因子3(FGF3)在舌癌中的表达水平及对舌鳞状细胞癌SCC-9细胞增殖及迁移的影响。方法收集2015年1月~2020年1月新疆医科大学第一附属医院明确诊断的40例舌鳞状细胞癌组织及对应癌旁组织。将舌磷癌细胞株SCC-9细胞分为空白组(正常培养72 h)、FGF3干预组(加入100 ng/mL FGF3进行干预后培养72 h)、FGFR抑制剂干预组(加入100 nM FGFR抑制剂后进行干预后培养72 h)。采用免疫组化检测舌鳞状细胞癌及癌旁组织FGF3的表达;采用划痕实验、CCK-8实验检测空白组、FGF3干预组、FGFR抑制剂干预组对舌鳞癌细胞迁移及增殖的影响。结果在40例舌鳞癌及对应癌旁组织中,FGF3高表达分别为90%及12.5%,两者具有显著的统计学差异(P<0.01);划痕实验组中,24 h时,空白组、FGF3干预组、FGFR抑制剂干预组划痕面积百分比分别为:(13.106±0.907)%、(7.515±0.733)%、(21.099±1.113)%;与空白组比较,FGF3干预组及FGFR抑制剂干预组划痕面积百分比差异均有统计学意义(P<0.05)。CCK-8实验的结果显示空白组、FGF3干预组、FGFR抑制剂干预组细胞的存活率为(100.000±4.026)%、(136.330±9.779)%、(83.199±4.954)%,与空白组比较,FGF3干预组及FGFR抑制剂干预组细胞的存活率差异均有统计学意义(P<0.05)。结论FGF3在舌鳞癌中高表达,促进舌鳞癌细胞系SCC-9细胞的增殖及迁移。Objective To investigate the expression of fibroblast growth factor 3(FGF3)in tongue cancer and its effect on the proliferation and migration of SCC-9 cells.Methods From January 2015 to January 2020,40 cases of tongue squamous cell carcinoma and corresponding adjacent tissues were definitely diagnosed in the First Affiliated Hospital of Xinjiang Medical University.The expression of FGF3 in 40 cases of tongue squamous cell carcinoma and adjacent tissues was detected by immunohistochemistry.The effects of blank group,FGF3 intervention group and FGFR inhibitor intervention group on the migration and proliferation of tongue squamous cell carcinoma cells were detected by scratch test and CCK-8 test.Results In 40 cases of tongue squamous cell carcinoma and corresponding adjacent tissues,the high expression of FGF3 was 90%and 12.5%.There was significant statistical difference between the two(P<0.01);in the scratch experimental group,at 24 h,the percentage of scratch area in the blank group,FGF3 intervention group and FGFR inhibitor intervention group were(13.106±0.907)%,(7.515±0.733)%and(21.099±1.113)%respectively.There were significant differences in the percentage of scratch area between FGF3 intervention group and blank group and between FGFR inhibitor intervention group and blank group(P<0.05).The results of CCK-8 experiment showed that the survival rates of cells in blank group,FGF3 intervention group and FGFR inhibitor intervention group were(100.000±4.026)%,(136.330±9.779)%and(83.199±4.954)%.There were significant differences between FGF3 intervention group and blank group,FGFR inhibitor intervention group and blank group(P<0.05).Conclusion FGF3 is highly expressed in tongue squamous cell carcinoma and promotes the proliferation and migration of tongue squamous cell carcinoma cell line SCC-9.

关 键 词：成纤维生长因子3 舌鳞状细胞癌 细胞增殖 细胞迁移 

分 类 号：R739.86[医药卫生—肿瘤]