微小RNA-454、微小RNA-132、液泡膜-ATP酶表达与结肠癌临床病理特征关联性分析  

Correlation of tissue microRNA-454, microRNA-132 and vesicle membrane-ATPase expression with clinicopathological characteristics of colon cancer patients

作　　者：孟利军 朱艳丽[1] 郭晓鹤[1] 蒯君 杨璐 杨芳 秦咏梅[1] MENG Lijun;ZHU Yanli;GUO Xiaohe;KUAI Jun;YANG Lu;YANG Fang;QIN Yongmei(Department of Gastroenterology,First Affiliated Hospital of Xinxiang Medical College,Xinxiang,Henan 453100,China)

机构地区：[1]新乡医学院第一附属医院消化内科,河南新乡453100

出　　处：《安徽医药》2022年第12期2469-2473,共5页Anhui Medical and Pharmaceutical Journal

基　　金：河南省医学科技公关计划项目(201702129)。

摘　　要：目的 探讨组织微小RNA(miR)-454、miR-132、液泡膜-ATP酶(V-ATPase)表达与结肠癌病人临床病理特征关联性。方法 选取2011年9月至2014年11月新乡医学院第一附属医院行手术切除的96例结肠癌组织标本、96例癌旁正常结肠组织标本、82例良性结肠病变标本为研究对象。以实时荧光定量逆转录聚合酶链反应(qRT-PCR)测定标本中miR-454、miR-132、V-ATPase mRNA表达,分析miR-454、miR-132、V-ATPase mRNA表达与结肠癌病人一般资料、临床病理参数的关系,随访5年,统计miR-454、miR-132、V-ATPase高表达、低表达病人3年、5年生存率。结果 结肠癌组织标本中miR-454表达69.86±7.29与V-ATPase mRNA表达5.42±0.41高于良性结肠病变组织10.74±2.03、2.79±0.38、癌旁正常结肠组织标本1.28±0.74、1.96±0.32,miR-132水平表达1.46±0.25低于良性结肠病变组织3.68±0.73、癌旁正常结肠组织标本4.59±1.24(P<0.05);中低分化、Ducks分期C~D期、有淋巴结转移结肠癌病人miR-454与V-ATPase mRNA表达高于高分化、Ducks分期为A~B期、无淋巴结转移病人,miR-132表达低于高分化、Ducks分期为A~B期、无淋巴结转移病人(P<0.05);miR-454、V-ATPase mRNA表达与组织分化程度呈显著负相关,与Ducks分期、淋巴结转移呈显著正相关,miR-132表达与组织分化程度呈显著正相关,与Ducks分期、淋巴结转移呈显著负相关(P<0.05);miR-454、V-ATPase mRNA高表达病人3年(9.23%、33.85%)、5年生存率(44.00%、28.00%)低于低表达(70.97%、61.29%)、(69.57%、55.10%)病人,miR-132高表达病人3年、5年生存率(70.59%、58.82%)高于低表达病人(48.39%、33.87%)(P<0.05)。结论 miR-454、V-ATPase mRNA表达与组织分化程度呈显著负相关,与Ducks分期、淋巴结转移呈显著正相关,miR-132表达与组织分化程度呈显著正相关,与Ducks分期、淋巴结转移呈显著负相关,可为结肠癌恶性化生物学行为特征、病理级别、预后判断提供客观依据。Objective To investigate the association of tissue microRNA(miR)-454,miR-132,and vesicular membrane-ATPase(VATPase) expression with the clinicopathological characteristics of patients with colon cancer.Methods Totally 96 colon cancer tissue specimens,96 paracancerous normal colon tissue specimens,and 82 benign colon lesion specimens,obtained from surgical resection at the First Affiliated Hospital of Xinxiang Medical College from September 2011 to November 2014,were selected for the study.The expressions of miR-454,miR-132 and V-ATPase mRNA in the specimens were measured by qRT-PCR,and the relationship between miR-454,miR-132 and V-ATPase mRNA expressions and general data and clinicopathological parameters of colon cancer patients was analyzed.Patients were followed up for 5 years and the 3-year and 5-year survival rates were calculated of the patients with high or low miR-454,miR-132,and V-ATPase expressions.Results The expressions of miR-454 and V-ATPase mRNA were higher in colon cancer tissue specimens than in benign colon lesion tissues,and normal colon tissue specimens adjacent to cancer [miR-454:69.86±7.29 vs.(10.74±2.03),(1.28±0.74);V-ATPase mRNA:(5.42±0.41) vs.(2.79±0.38),(1.96±0.32)],and miR-132 expression was lower than that of benign colon lesion tissues and normal colon tissue specimens adjacent to cancer(1.46±0.25 vs.3.68±0.73,4.59±1.24;P<0.05).The expressions of miR-454 and V-ATPase mRNA were higher in patients with moderately low-differentiated,Ducks stage C to D,colon cancer with lymph node metastasis than in patients with highly differentiated,Ducks stage A to B,and no lymph node metastasis,and miR-132 expression was lower than that in the patients with highly differentiated,Ducks stage A to B,and no lymph node metastasis(P<0.05).miR-454 and V-ATPase mRNA expressions were significantly negatively correlated with the degree of tissue differentiation and significantly positively correlated with Ducks staging and lymph node metastasis.miR-132 expression was significantly positively correlat

关 键 词：结肠肿瘤 微小RNA-454 微小RNA-132 液泡膜-ATP酶 病理特征 

分 类 号：R735.35[医药卫生—肿瘤]