长链非编码RNA CASC15在胶质瘤细胞自噬过程中的调控作用  

Regulation of Long Non-coding RNA CASC15 on Autophagy via ATG3 in Glioma Cells

作　　者：庞星一 王贺[1] PANG Xingyi;WANG He(Affiliated Hospital of Beihua University,Jilin 132011,China)

机构地区：[1]北华大学附属医院,吉林吉林132011

出　　处：《北华大学学报（自然科学版）》2022年第5期596-601,共6页Journal of Beihua University（Natural Science）

基　　金：吉林省卫生与健康青年科技骨干培养计划项目(2020Q007).

摘　　要：目的探讨长链非编码RNA CASC15在胶质瘤细胞自噬过程中的调控作用.方法应用TCGA和GTEx数据库分析CASC15在胶质瘤组织与正常组织中的表达情况,构建过表达细胞系;应用PCR法检测CASC15在不同细胞系中的表达及转染后的表达情况,CCK8检测CASC15对细胞的生长能力的影响,Western blot检测自噬相关蛋白LC3和P62的表达.结果CASC15在胶质瘤组织及胶质瘤细胞系中高表达,并可促进U251和U87胶质瘤细胞的生长;CASC15能够增加胶质瘤细胞LC3Ⅱ/LC3Ⅰ的比率,并降低P62的表达;CASC15还能促进ATG3的表达,但ATG5和ATG7无变化.结论CASC15可促进胶质瘤细胞生长,并激活胶质瘤细胞自噬.Objective To explore the regulation role of long non-coding RNA CASC15 on autophagy in glioma cells.Method TCGA and GTEx database were used to analyze the expression of CASC15 in glioma tissues,and CASC15-overexpressed cell lines were constructed.PCR was used to detect the expression of CASC15 in different cell lines after transfection with CASC15;CCK8 was used to detect the effect of CASC15 on cell proliferation,and Western blot was used to detect the expression of autophagy-related markers LC3 and P62.Results CASC15 was highly expressed in glioma tissues and increased in various glioma cell lines.CASC15 promotes glioma cell growth.CASC15 promotes LC3Ⅱ/LC3Ⅰrate in glioma cells and it also decreased the P62 expression.CASC15 enhances the expression of ATG3 in glioma cells.Conclusion CASC15 promotes the glioma cell proliferation and activates autophagy of glioma cells.

关 键 词：长链非编码RNA CASC15 自噬 胶质瘤 

分 类 号：R739.4[医药卫生—肿瘤]