响应面法优化混菌发酵肉鹅青贮饲料工艺研究  

Optimization of Mixed Fermentation of Silage for Goose by Response Surface Methodology

作　　者：裴朝东[1] PEI Chaodong(Guangxi Vocational College of Technology and Business,Guangxi Nanning 530007,China)

机构地区：[1]广西工商职业技术学院,广西南宁530007

出　　处：《饲料工业》2022年第21期28-34,共7页Feed Industry

基　　金：广西高校中青年教师科研基础能力提升项目[2021KY1343];广西工商职业技术学院2022年院级项目[XY2022ZXYB13]。

摘　　要：试验以嗜酸乳杆菌(Lactobacilltus acidlophilus)、枯草芽孢杆菌(Bacillus subtilis)、酿酒酵母(Saccharomyces cerevisiae)混菌发酵肉鹅青贮饲料为研究对象,以嗜酸乳杆菌活菌数为指标,应用响应面法(RSM)对其工艺进行优化。试验采用Design Expert 13.0.1.0软件中的Box-Behnken响应面优化设计试验方案,共配制29组组方进行试验。通过测定嗜酸乳杆菌的活菌数变化,利用二次响应曲面回归方法建立数学模型,分析各因素对活菌数变化的影响。试验结果表明:当稻壳为148.663 g、酒糟为88.657 g、玉米粉为28.448 g、象草为280.182 g时,在特定的温度条件下(36±1)℃和指定的接种量(4%),经过混合发酵72 h以上,嗜酸乳杆菌活菌数可达44.92×107 CFU/g。The aim of this study was to optimize the mixed fermentation of silage for goose by Lactobacil⁃ltus acidlophilus,Bacillus subtilis and Saccharomy⁃ces cerevisiae,which was applied to the response surface methodology(RSM).The Box-Behnken in Design Expert 13.0.1.0 was used in 29 groups.By measuring the changing of Lactobacillus acidophi⁃lus,a quadratic regression model was established in order to analyze the effect of each factor on bacterial counts.The results showed that the optimal fermentation component was as the followings:rice-husk was 148.663 g,rosacea was 88.657 g,corn meal was 28.448 g and elehpant grass was 280.182 g.Under these contitions the number of Lactobacillus acidophilus could reach 44.92×10^(7) CFU/g,which the temperature was(36±1)℃,the fermentation time was 72 hours and the inoculum was 4%.

关 键 词：嗜酸乳杆菌 混菌发酵 响应面优化 肉鹅饲料 青贮饲料 

分 类 号：S816.6[农业科学—饲料科学]