蟾蜍灵对人胃黏膜上皮细胞GES-1的毒性研究  

The Cytotoxicity of Bufalin on Human Gastric Epithlial Cell GES-1

作　　者：刘玉玲 刘利 邓佳丽 郭祉良 LIU Yuling;LIU Li;DENG Jiali;GUO Zhiliang(Fenyang College of Shanxi Medical University,Fenyang 032200,China;Fenyang Hospital of Shanxi,Fenyang 032200,China)

机构地区：[1]山西医科大学汾阳学院,山西汾阳032200 [2]山西省汾阳医院,山西汾阳032200

出　　处：《生物化工》2022年第5期103-105,109,共4页Biological Chemical Engineering

基　　金：山西省科技厅青年科技研究基金(No.20210302124580);国家级大学生创新创业训练计划项目(No.20221568);山西省高等学校大学生创新创业训练计划重点项目(No.S202117114004);山西省高等学校大学生创新创业训练计划项目(No.2020785;No.FDC201903)。

摘　　要：目的:研究不同浓度蟾蜍灵对人体胃黏膜上皮细胞GES-1的毒性作用。方法:用不同浓度(0 nmol/L、1 nmol/L、10 nmol/L、25 nmol/L、50 nmol/L和100 nmol/L)蟾蜍灵作用于GES-1细胞24 h、48 h和72 h,通过四甲基偶氮唑盐(MTT)法检测蟾蜍灵对GES-1细胞增值的影响;再分别以0 nmol/L、25 nmol/L、50 nmol/L和100 nmol/L蟾蜍灵处理GES-1细胞48 h,在光学显微镜下观察GES-1细胞形态变化,运用荧光探针DCFH-DA法在荧光显微镜下观察细胞内绿色荧光强度。结果:不同浓度(0~100 nmol/L)蟾蜍灵处理GES-1细胞24 h时,对细胞无杀伤作用(与对照组0 nmol/L相比,P>0.05),但在48 h和72 h时,蟾蜍灵抑制GES-1细胞的生长,且呈浓度依赖性(与对照组0 nmol/L相比,P<0.05),同时诱导细胞内产生大量的ROS。结论:蟾蜍灵可以引起人胃黏膜上皮细胞GES-1的损伤,并诱导细胞内产生大量的ROS,本实验的结果将为蟾蜍灵在临床中的应用提供实验依据。Objective:To study the toxic effects of Bufalin with different concentrations on human gastric epithelial cells GES-1.Methods:GES-1 cells are first treated with Bufalin at different dose(0 nmol/L,1 nmol/L,10 nmol/L,25 nmol/L,50 nmol/L,100 nmol/L)for 24 h,48 h and 72 h,and the proliferation of GES-1 cells is detected by MTT method.Then the GES-1 cells is stimulated by 0 nmol/L,25 nmol/L,50 nmol/L,100 nmol/L Bufalin for 48 h,the morphology of GES-1 cells is observed by light microscope,and the green fluorescence intensity of cells is observed by fluorescence microscope DCFH-DA under fluorescence microscope.Results:Bufalin has no killing effect on GES-1 cells at different concentrations(0~100 nmol/L)with 24 h treatment(P>0.05 compared with 0 nmol/L in the control group),but at 48 h and 72 h,Bufalin inhibit the growth of GES-1 cells in a concentration-dependent manner(P<0.05,compared with 0 nmol/L in the control group).Conclusion:Bufalin can cause GES-1 cells injury,and induce a large number of ROS production.The results of this study will provide experimental basis for the clinical application of Bufalin.

关 键 词：蟾蜍灵 人胃黏膜上皮细胞 细胞增值 活性氧 

分 类 号：R735.2[医药卫生—肿瘤]