基于线粒体COⅠ基因滁州地区克氏原螯虾养殖群体遗传多样性分析  

Analysis of Genetic Diversity of P.clarkii Culture Population in Chuzhou Based on Mitochondrial COⅠ Gene

作　　者：余红喜 刘帆 朱国美 任信林 刘鑫鑫 凌武海 苏时萍[2] YU Hong-xi;LIU Fan;ZHU Guo-mei(Chuzhou Agricultural and Rural Technology Promotion Center,Chuzhou,Anhui 239000;College of Animal Science and Technology,Anhui Agricultural University,Hefei,Anhui 230036)

机构地区：[1]滁州市农业农村技术推广中心,安徽滁州239000 [2]安徽农业大学动物科技学院,安徽合肥230036

出　　处：《安徽农业科学》2022年第21期111-113,125,共4页Journal of Anhui Agricultural Sciences

基　　金：滁州市乡村振兴农业发展专项资金项目;滁州市科技计划项目;滁州市第一批“113”产业创新团队;安徽省水产产业体系([2021]711)。

摘　　要：[目的]了解安徽滁州市克氏原螯虾养殖群体的遗传学特征。[方法]基于线粒体细胞色素氧化酶亚基I(COⅠ)基因序列,采用直接测序法分析8个克氏原螯虾养殖群体的遗传多样性变化、群体结构和遗传分化情况。[结果]8个养殖群体共有25个变异位点,包括15个简约信息位点和10个单碱基变异位点;序列中(A+T)的含量(67.0%)明显高于(G+C)的含量(33.0%),具有明显的(A+T)碱基偏倚。遗传多样性分析显示,全部样本的单倍型多样性指数为0.373 14,核苷酸多样性指数为0.001 72,呈现低单倍型多样性和低核苷酸多样性,且单倍型之间不具有明显的地理分化特征,不同养殖群体之间混杂在一起。群体间遗传分化系数F为-0.023 56~0.122 14,分化程度极低。[结论]滁州市8个克氏原螯虾养殖群体的遗传多样性处于较低水平,建议扩大亲本选种范围,避免近亲繁殖和盲目交换亲本。[Objective]To research the genetic characteristics of farmed Procambarus clarkii population in Chuzhou City, Anhui Province. [Method]We compared nucleotide sequences of mitochondrial cytochrome oxidase subunit I(COⅠ) gene of mitochondrial DNA(mtDNA) from eight farmed populations to investigate changes in genetic diversity, population structure and genetic differentiation. [Result]The results showed that 8 populations had 25 mutation sites, including 15 parsimony information sites and 10 single-base mutation sites;the content of(A + T)(67.0%) was significantly higher than(G + C)(33.0%),with obvious(A+T) base bias. Genetic diversity analysis showed that the haplotype diversity index of all samples was 0.373 14,and the nucleotide diversity index was 0.001 72. The 8 populations showed low haplotype diversity and oligonucleotide diversity, moreover, the haplotypes do not have obvious geographic differentiation characteristics, but are mixed together among those farmed groups. The coefficient of genetic differentiation among populations Fwas-0.023 56-0.122 14,and the degree of differentiation was extremely low. [Conclusion]The genetic diversity of 8 populations of farmed Procambarus clarkii population in Chuzhou City is at a relatively low level. It is suggested to expand the selection range of parents and avoid inbreeding and blind exchange of parents.

关 键 词：克氏原螯虾 线粒体DNA COⅠ基因 遗传多样性 

分 类 号：S966.12[农业科学—水产养殖]