G-CSF促进晶状体上皮细胞增殖和纤维化的影响  

Effects of G-CSF on proliferation and fibrosis of lens epithelial cells

作　　者：景瑞花 王韵卿 乔鑫利 薛菲 马波[2] JING Ruihua;WANG Yunqing;QIAO Xinli;XUE Fei;MA Bo(Department of Ophthalmology,The Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004;Department of Ophthalmology,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China)

机构地区：[1]西安交通大学第二附属医院眼科,陕西西安710004 [2]西安交通大学第一附属医院眼科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2022年第6期840-844,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81800812)。

摘　　要：目的粒细胞集落刺激生长因子(G-CSF)近年来被证明在后发性白内障(PCO)晶状体后囊膜组织中表达。本研究旨在探讨G-CSF是否在PCO中发挥作用。方法采用浓度梯度重组G-CSF蛋白处理人晶状体上皮细胞(HLEC-B3),筛选有效、合适的作用浓度。Western blotting检测G-CSF处理HLEC-B3细胞后细胞外基质(ECM)合成、上皮间质转分化(EMT)标志基因的作用。划痕实验和Transwell实验检测G-CSF处理对HLEC-B3细胞迁移和侵袭的作用。结果80μg/L G-CSF可以促进HLEC-B3细胞增殖。G-CSF处理HLEC-B3细胞48 h后EMT和ECM合成标志基因的表达随时间明显上调。G-CSF处理HLEC-B3细胞48 h可以明显促进其侵袭,同样,G-CSF也可以明显诱导细胞迁移。结论G-CSF可以促进HLEC-B3细胞增殖、迁移、侵袭及EMT和ECM合成,可能参与了PCO的发生,抑制G-CSF表达可能是控制PCO的新策略。Objective In recent years,granulocyte colony stimulating growth factor(G-CSF)has been proved to be expressed in the posterior capsule of the lens of posterior capsular opacification(PCO),but its specific role remains unclear.The purpose of this study was to explore whether G-CSF plays a role in PCO.Methods First,human lens epithelial cells(HLEC-B3)were treated with different concentration of recombinant G-CSF protein and screened for effective appropriate concentration.Then,Western blotting was used to detect the effects of extracellular matrix(ECM)synthesis and epithelial mesenchymal transdifferentiation(EMT)marker genes after G-CSF treatment on HLEC-B3 cells.Finally,the effects of G-CSF treatment on the migration and invasion of HLEC-B3 cells were detected by scratch experiment and Transwell test.Results G-CSF at 80μg/L could promote the proliferation of HLEC-B3 cells.After G-CSF was treated for 48 h,the expressions of EMT and ECM synthesis marker genes in HLEC-B3 cells were significantly upregulated with time.G-CSF could significantly promote HLEC-B3 cell invasion after 48 h induction.Similarly,G-CSF could also significantly induce cell migration compared to the CTRL group.Conclusion G-CSF can promote the proliferation,migration and invasion of HLEC-B3 cells,as well as EMT and ECM synthesis,which might be involved in the occurrence of PCO.Inhibiting the expression of G-CSF may be a new strategy for PCO prevention.

关 键 词：后发性白内障 粒细胞集落刺激因子 上皮间质转分化 细胞外基质 

分 类 号：R774[医药卫生—眼科]