槲皮素金属配合物抑制胆囊癌细胞增殖、侵袭及增强化疗敏感性的机制分析  

The Mechanism of Quercetin Metal Complex Inhibiting Proliferation,Invasion and Enhancing Chemosensitivity of Gallbladder Cancer Cells

作　　者：刘涛[1] 白浪[1] 高永涛[1] LIU Tao;BAI Lang;GAO Yongtao(Affiliated Hospital of Yan'an University,Yan'an,716000)

机构地区：[1]延安大学附属医院,716000

出　　处：《实用癌症杂志》2022年第11期1741-1744,共4页The Practical Journal of Cancer

摘　　要：目的观察槲皮素金属配合物抑制囊癌细胞增殖、侵袭,增强吉西他滨化疗敏感性的机制。方法采用CCK8法、细胞克隆形成实验检测槲皮素金属配合物对胆囊癌GBC-SD细胞增殖的影响;划痕实验和Transwell体外侵袭实验检测细胞侵袭迁移能力的影响;RT-PCR法检测槲皮素金属配合物对GBC-SD细胞内人平衡型核苷转运体1(Human Equilibrative Nucleoside Transporter-1,hENT-1)mRNA表达的影响。结果160μmol/L的Co(Que)_(2)对GBC-SD细胞的抑制率达(83.5±15.8)%,培养120 h后Co(Que)_(2)对GBC-SD细胞的抑制率达(87.7±12.6)%;Co(Que)_(2)+GEM组细胞迁移数目最少(95±16.7),Co(Que)_(2)+GEM组hENT-1 mRNA的表达为(0.61±0.15)。结论槲皮素金属配合物抑制胆囊癌细胞的增殖、侵袭迁移,通过上调hENT-1增强吉西他滨对胆囊癌细胞化疗敏感性。Objective To observe the inhibitory effect of quercetin metal complex on the proliferation and invasion of cystic carcinoma cells,and the mechanism of enhancing the chemosensitivity of Gemcitabine.Methods CCK8 assay and colony formation assay were used to detect the effect of quercetin on GBC-SD cell proliferation;Scratch test,Transwell invasion test and RT-PCR were used to detect the ability of cell invasion and migration,and the effect of quercetin metal complex on the expression of human balanced nucleoside transporter-1(hENT-1)mRNA in GBC-SD cells.Results The inhibition rate of 160μmol/L CO(Que)_(2) on GBC-SD cells was(83.5±15.8)%,and the inhibition rate of CO(Que)_(2) on GBC-SD cells was(87.7±12.6)%after 120h culture;The migration number of cells in CO(Que)_(2)+GEM group was the least(95±16.7),and the expression of hENT-1 mRNA in CO(Que)_(2)+GEM group was(0.61±0.15).Conclusion Quercetin metal complexes inhibit the proliferation,invasion and migration of gallbladder cancer cells,and enhance the chemosensitivity of gemcitabine to gallbladder cancer cells by up regulating hENT-1.

关 键 词：槲皮素 胆囊癌 吉西他滨 

分 类 号：R735.8[医药卫生—肿瘤]