COX-2和Ki-67表达与下咽癌临床病理参数的关系分析  

Relationship Between the Expression of COX-2 and Ki-67 and Clinicopathological Parameters of Hypopharyngeal Carcinoma

作　　者：刘晨 刘稳 LIU Chen;LIU Wen(Xuzhou Central Hospital,Xuzhou,221000)

机构地区：[1]江苏省徐州市中心医院,221000 [2]徐州医科大学附属医院,221000

出　　处：《实用癌症杂志》2022年第11期1787-1790,共4页The Practical Journal of Cancer

摘　　要：目的研究下咽癌组织中环氧合酶-2(COX-2)和Ki-67的表达情况及与其肿瘤临床病理参数的关系及相应临床意义。方法采用免疫组织化学染色法(SP法),检测36例下咽癌组织与癌旁正常组织中COX-2和Ki-67的表达,并采用Western-blot方法再次验证36例下咽癌组织与癌旁正常组织中COX-2的定量,结合肿瘤对应的各项病理参数进行综合分析。结果免疫组化显示36例下咽癌组织中COX-2和Ki-67阳性表达率分别为77.8%(28/36)和83.3%(30/36),下咽癌旁正常组织中COX-2和Ki-67的阳性率仅为33.3%(12/36)和19.4%(7/36)。较高T分期的下咽癌或存在淋巴结转移时,肿瘤组织中COX-2和Ki-67阳性率显著升高(P均<0.05)。Western-blot验证COX-2在下咽癌组织中表达水平明显高于癌旁组织。结论COX-2和Ki-67在下咽癌组织中的高度表达,且两者与下咽癌的临床病理特征密切相关,其中COX-2在癌组织中表达量显著高于癌旁组织,表明COX-2和Ki-67可能作为下咽鳞状细胞癌早期诊断及预后判断的分子指标之一,联合检测COX-2和Ki-67可能对判断下咽鳞癌的进展及预后有一定参考价值。Objective To investigate the relationship between the expression of cyclooxygenase-2(COX-2)and Ki-67 and the clinical significance of hypopharyngeal carcinoma tissues.Methods The expressions of COX-2 and Ki-67 in 36 cases of hypopharyngeal carcinoma and paracancerous tissues were detected by immunohistochemical staining(SP method).The quantification of COX-2 in 36 cases of hypopharyngeal carcinoma and paracancerous tissues was verified by Western blot.And the relationship of expression of COX-2 and Ki-67 linked with clinical pathological characters were analyzed.Results Immunohistochemistry showed that the positive rates of COX-2 and Ki-67 in 36 cases of hypopharyngeal carcinoma were 77.8%(28/36)and 83.3%(30/36),respectively.The positive rates of COX-2 and Ki-67 in paracancerous tissues were only 33.3%(12/36)and 19.4%(7/36).When hypopharyngeal carcinoma with higher T stage or lymph node metastasis,the number of COX-2 and Ki-67 positive cells in tumor tissue increased,and the positive rate increased significantly(P<0.05).Western-blot showed that the expression of COX-2 in hypopharyngeal carcinoma was significantly higher than that in paracancerous tissues.Conclusion COX-2 and Ki-67 were highly expressed in hypopharyngeal carcinoma,and they were closely related to the clinicopathological features of hypopharyngeal carcinoma.The expression of COX-2 in cancer tissues was significantly higher than that in paracancerous tissues,indicating that the combined COX-2 and Ki-67 may be one of the molecular indicators for the early diagnosis and prognosis of hypopharyngeal carcinoma,and the combined detection of them may have a certain reference value for judging the progression and prognosis of hypopharyngeal carcinoma.

关 键 词：COX-2 KI-67 下咽鳞状细胞癌 

分 类 号：R739.63[医药卫生—肿瘤]