CD36在非小细胞肺癌中的表达及与临床病理特征的关系  

Expressions of CD36 in non-small cell lung cancer and their relationships with clinicopathological features

作　　者：杨志强 钱立勇 金丹雯 李婷玉 温媛媛 何慧 YANG Zhiqiang;QIAN Liyong;JIN Danwen;LI Tingyu;WEN Yuanyuan;HE Hui(Department of Respiratory Medicine,Zhoushan Hospital,Zhejiang,Zhoushan 316021,China;Department of Pathology,Zhoushan Hospital,Zhejiang,Zhoushan 316021,China)

机构地区：[1]舟山医院呼吸与危重症医学科,浙江舟山316021 [2]舟山医院病理科,浙江舟山316021

出　　处：《中国现代医生》2022年第32期5-11,共7页China Modern Doctor

基　　金：浙江省医药卫生科技计划项目(2019KY739);舟山市医药卫生科技计划项目(2018G03)。

摘　　要：目的探索人类白细胞分化抗原36(cluster of differentiation 36,CD36)与非小细胞肺癌临床病理特征及生存预后之间的相关性,并分析其与肺癌免疫浸润水平的关系。方法利用UALCAN数据库挖掘分析工具(http://ualcan.path.uab.edu)分析CD36在肺鳞状细胞和肺腺癌组织和癌旁组织中的表达差异及预后相关性,选取2017年1月至2020年12月舟山医院收治的非小细胞肺癌患者49例,利用实时荧光定量聚合酶链反应(reverse transcription quantitative polymerase chain reaction,RT–qPCR)和免疫组织化学法分析CD36在非小细胞肺癌中的表达及其与临床病理特征之间的关系。结果公共数据库分析及RT–qPCR结果均显示CD36 mRNA在癌旁组织的表达量显著高于癌组织,差异均有统计学意义(P<0.01)。CD36的高表达与肺鳞状细胞癌的预后呈负相关(P<0.05),与肺腺癌的预后不具有相关性(P>0.05)。免疫组织化学结果显示,CD36蛋白表达量与非小细胞肺癌病理类型相关(x^(2)=9.764,P=0.008),与肺膜侵犯和淋巴结转移情况不相关(P>0.05)。CD36表达与肺鳞状细胞癌和肺腺癌的CD8+T细胞、巨噬细胞、嗜中性粒细胞、树突状细胞的免疫浸润水平呈正相关(P<0.05)。结论CD36可作为肺鳞状细胞癌的生存预后标志物,CD36蛋白在不同病理类型的肺癌中表达有差异,CD36表达与肺鳞状细胞癌和肺腺癌肿瘤免疫浸润水平均有相关性。Objective To investigate the expression of cluster of differentiation 36(CD36)in non–small cell lung cancer and their relationships with clinicopathological features and prognosis.Methods The CD36 mRNA expression differences between lung squamous cell and lung adenocarcinoma tissues and tissue adjacent to carcinoma were analyzed by UALCAN database(http://ualcan.path.uab.edu),and their relationships with prognosis was also analyzed.A total of 49 patients with non–small cell lung cancer who were treated in Zhoushan Hospital from January 2017 to December 2020 were selected.The expression of CD36 in non–small cell lung cancer were detected by reverse transcription quantitative polymerase chain reaction(RT–qPCR)and immunohistochemistry,and their relationship with clinicopathologic feature were analyzed.Results Public database analysis and RT–qPCR results both showed that the expression level of CD36mRNA in para–cancer tissues were significantly higher than those in cancer tissues(P<0.01).The high expression of CD36 was negatively correlated with the prognosis of lung squamous cell carcinoma(P<0.05),while it was not correlated with the prognosis of lung adenocarcinoma(P>0.05).Immunohistochemical results showed that the expression level of CD36 protein was correlated with the pathological type of NSCLC(x^(2)=9.764,P=0.008),but not with the invasion of lung membrane and lymph node metastasis(P<0.05).CD36 expression was positively correlated with immune infiltration of CD8^(+)T cells,macrophages,neutrophils and dendritic cells in lung squamous cell carcinoma and lung adenocarcinoma(P<0.05).Conclusion CD36 can be used as a prognostic marker of lung squamous cell carcinoma, CD36 protein expression was different in different pathological types of lung cancer, CD36 expression was related to tumor immune invasion level of lung squamous cell carcinoma and lung adenocarcinoma.

关 键 词：人类白细胞分化抗原36 非小细胞肺癌 预后 免疫浸润水平 

分 类 号：R734.2[医药卫生—肿瘤]