3种激光处理对微弧氧化钛板微结构和牙周膜干细胞成骨活性的影响  

Effect of Diode,Er,and Nd:YAG Laser Irradiation on Microstructure of Microarc Oxidation Titanium Disc and Osteogenesis Activity of Periodontal Ligament Stem Cells

作　　者：黄文霞 左起亮 张秋芳 聂晶 韩阳平 许铭炎 詹暶 HUANG Wenxia;ZUO Qiliang;ZHANG Qiufang;NIE Jing;HAN Yangping;XU Minyan;ZHAN Xuan(Stomatological Hospital of Xiamen Medical College,Xiamen 361000,China;Xiamen Key Laboratory of Stomatological Disease Diagnosis and Treatment,Xiamen 361000,China;School and Hospital of Stomatology,Fujian Medical University,Fuzhou 350000,China;Key Laboratory of Stomatology,Fujian Provincial University,Fuzhou 350000,China)

机构地区：[1]厦门医学院附属口腔医院,福建厦门361000 [2]厦门市口腔疾病诊疗重点实验室,福建厦门361000 [3]福建省高校口腔医学重点实验室,福建福州350000 [4]福建医科大学附属口腔医院,福建福州350000

出　　处：《口腔医学研究》2022年第11期1052-1057,共6页Journal of Oral Science Research

基　　金：福建省高校口腔医学重点实验室开放性课题(编号:2019kq002)。

摘　　要：目的:研究半导体(Diode)、铒(Er)和Nd:YAG激光照射对微弧氧化(microarc oxidation,MAO)钛板表面的微结构和牙周膜干细胞成骨活性的影响。方法:采用Diode、Er和Nd:YAG激光分别对MAO钛板表面照射,扫描电镜观察MAO钛表面以及人牙周膜干细胞(human periodontal ligament stem cells,hPDLSCs)的形貌,X射线电子衍射光谱(X-ray diffraction,XRD)分析MAO钛表面的晶相,CCK-8法检测细胞的增殖活性A值,RT-qPCR法检测细胞的Runx2、ALP、OCN和OSX的表达水平。结果:扫描电镜和XRD显示经3种激光照射后MAO钛表面的形貌和晶相均发生改变;经Nd:YAG激光照射的MAO钛表面显著促进hPDLSCs的Runx2和ALP的表达(P<0.001),但其A值下降(P<0.05);经Er激光照射的MAO钛表面促进细胞的ALP基因表达(P<0.005);经Diode激光照射的MAO钛表面的细胞A值以及成骨基因的表达量均减少。结论:经Er和Nd:YAG激光照射的MAO钛表面可有效促进hPDLSCs成骨分化,而Diode激光照射的MAO钛表面不利于细胞的增殖和分化。Objective:To study the effect of diode,Er,and Nd:YAG laser irradiation on the microstructure of microarc oxidation(MAO)titanium disks and osteogenesis activity of periodontal ligament stem cells.Methods:The diode,Er,and Nd:YAG laser were applied to irradiate the MAO titanium disks,after which scanning electron microscope(SEM)was used to observe the morphology of discs and the seeded human periodontal ligament stem cells(hPDLSCs).Crystal phase of MAO titanium disc was analyzed by X-ray diffraction(XRD).The proliferation of hPDLSCs was determined by CCK-8.The expressions of Runx2,ALP,OCN,and OSX gene in hPDLSCs were detected by RT-qPCR.Results:SEM images and XRD spectrum revealed that diode,Er,and Nd:YAG laser could alter the topography and crystal phases of MAO titanium discs.The expressions of Runx2 and ALP genes of hPDLSCs on the surface of MAO titanium disks irradiated by Nd:YAG laser were significantly increased(P<0.001),but the proliferation was reduced(P<0.05).The expression of ALP gene of cells on the surface of disks irradiated by Er laser was increased(P<0.005);however,the proliferation and osteoblastic gene expression of hPDLSCs on the disks irradiated by Diode laser were decreased.Conclusion:Irradiation by Er and Nd:YAG laser could effectively promote the osteogenic differentiation of hPDLSCs on the MAO titanium disks,however,irradiation by diode laser deteriorated the osteogenic differentiation and proliferation of cells.

关 键 词：半导体激光 铒激光 Nd:YAG激光 微弧氧化钛种植体 人牙周膜干细胞 

分 类 号：R781.05[医药卫生—口腔医学]