嗜酸细胞浸润食管固有肌层诱导小鼠贲门失弛缓症样食管运动障碍  

Eosinophilic infiltration into the muscularis propria of the esophagus induces achalasialike esophageal dyskinesia in mice

作　　者：张凌云 赵威[1] ZHANG Ling-yun;ZHAO Wei(Department of Gastroenterology and Hepatology,General Hospital,Tianjin Medical University,Tianjin 300052,China)

机构地区：[1]天津医科大学总医院消化科,天津300052

出　　处：《天津医科大学学报》2022年第6期609-614,共6页Journal of Tianjin Medical University

基　　金：天津市科委基金(20JCYBJC00450,20JCQNJC00560)。

摘　　要：目的:评估食管固有肌层嗜酸性粒细胞浸润对小鼠食管运动的影响。方法:通过长期卵白蛋白(OVA)刺激建立食管肌层嗜酸性粒细胞浸润小鼠模型。比较模型组(n=30)和对照组(n=20)固有肌层的嗜酸性粒细胞和肌层神经元的数量以及食管运动参数,如食管下括约肌压力(LESP)和食管排空。此外,比较模型组各时间点食管的组织学和运动情况。结果:与对照组相比,模型组食管运动功能严重恶化,与贲门失驰缓(AC)食管运动异常相似,肌层嗜酸性粒细胞增加(t=4.587,P<0.0001),肌层神经元减少(t=-4.283,P<0.0001)。随着OVA刺激时间的延长,模型组食管动力障碍加重(第28天时,LESP、食管通过时间、贲门通过时间P<0.05),食管肌层嗜酸性粒细胞增多(第28天时,t=4.429,P=0.0003),肌间神经元减少(第28天时,t=-4.328,P=0.0004)。此外,嗜酸性粒细胞浸润与食管肌间神经元数量呈负相关(r=0.531,P=0.026)。结论:长期OVA刺激可引起食管固有肌层嗜酸性粒细胞浸润、肌间神经元减少和食管动力障碍,食管肌层嗜酸性粒细胞浸润可能在AC的发病机制中起重要作用。Objective:To evaluate the effect of eosinophil infiltration in myenteric propria of esophaguson esophageal motility in mice.Methods:A mouse model of eosinophilic infiltration in esophageal muscularis was established by long-term ovalbumin(OVA)stimulation.The numbers of eosinophils and muscularis neurons in the myenteric propria and esophageal motility parameters such as lower esophageal sphincter pressure(LESP)and esophageal emptying were compared between the model group(n=30)and the control groups(n=20).In addition,the histology and motility of the esophagus at each time point in the model group were compared.Results:Compared with the control group,the esophageal motor function of the model group was severely deteriorated,similar to the abnormal esophageal motility of the achalasia(AC),the muscle layer eosinophils increased(t=4.587,P<0.0001),and the muscle layer neurons decreased(t=-4.283,P<0.0001).With the prolongation of OVA stimulation time,the esophageal motility disorder aggravated in the model group(on the 28th day,LESP,esophageal transit time,and cardiac transit time,P<0.05),and the eosinophilia in the esophageal muscle layer increased(t=4.429,P=0.0003 on day 28),myenteric neurons decreased(t=-4.328,P=0.0004 on day 28).In addition,eosinophil infiltration was negatively correlated with the number of esophageal myenteric neurons(r=0.531,P=0.026).Conclusion:Long-term OVA stimulation can induce eosinophil infiltration in the esophageal muscularis propria,decrease of myenteric neurons and esophageal motility disorders,and eosinophilic infiltration of the esophageal muscularis may play an important role in the pathogenesis of AC.

关 键 词：贲门失弛缓症 嗜酸性粒细胞 神经元 

分 类 号：R573.7[医药卫生—消化系统]