持续静压力作用下牙周膜细胞骨保护素、核因子-κB受体活化因子配体表达的增龄性变化  

Aging effect of osteoprotegerin and receptor activator of nuclear factor-κB ligand expression in human periodontal ligament cells under continuous static pressure

作　　者：吴洁 崔占琴[1] 韩瑜 李文静[3] Wu Jie;Cui Zhanqin;Han Yu;Li Wenjing(Dept.of Orthodontics,The Second Hospital of Hebei Medical University,Shijiazhuang 050000,China;Dept.of Stomatology,Shijiazhuang People’s Hospital,Shijiazhuang 050011,China;Dept.of Oral Medicine,The Second Hospital of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院口腔正畸科,石家庄050000 [2]石家庄市人民医院口腔科,石家庄050011 [3]河北医科大学第二医院口腔内科,石家庄050000

出　　处：《华西口腔医学杂志》2022年第6期654-661,共8页West China Journal of Stomatology

基　　金：河北省政府资助临床优秀人才项目(冀财社[2017]46号)。

摘　　要：目的通过细胞实验观察持续静压力作用下人牙周膜细胞(HPDLCs)表达骨保护素(OPG)、核因子-κB受体活化因子配体(RANKL)的增龄性变化。方法组织块法原代培养HPDLCs,分为A组(13~18岁)、B组(19~29岁)、C组(30~44岁)。CCK-8检测HPDLCs的增殖能力,β-半乳糖苷酶染色检测HPDLCs衰老情况。3组细胞均分别给予0、1.5、3、6、12、24、48、72 h体外持续静压力机械刺激,酶联免疫吸附测定法检测所提上清液中OPG、RANKL的表达。结果体外持续静压力刺激后,牙周膜细胞OPG表达水平随加力时间及年龄增长而增加(P<0.01)。RANKL表达水平随加力时间延长而增加,随年龄增长而下降(P<0.01)。OPG/RANKL比值随加力时间呈先下降后增加趋势,且随年龄增长而增加(P<0.01)。结论体外持续静压力作用下,增龄因素可上调牙周膜细胞OPG表达及OPG/RANKL比值,下调RANKL表达。Objective The expression of osteoprotegerin(OPG)and receptor activator of nuclear factor-κB ligand(RANKL)in human periodontal ligament cells(HPDLCs)was investigated by cell culture under continuous static pressure.Methods HPDLCs were primarily cultured by tissue explant method and divided into three groups:group A(13-18 years old),group B(19-29 years old),and group C(30-44 years old).CCK-8 was used to detect the proliferation of HPDLCs.The senescence of HPDLCs was detected by senescence-associatedβ-galactosidase staining.Cells in the three groups were respectively given 0,1.5,3,6,12,24,48,and 72 h of continuous static pressure in vitro.The expression of OPG and RANKL in the supernatant was detected by enzyme-linked immunosorbent assay.Results After continuous static pressure in vitro for stimulation,the expression of OPG and RANKL changed.The expression of OPG increased with time and age(P<0.01).The expression of RANKL increased with time and decreased with age(P<0.01).The ratio of OPG/RANKL initially decreased,increased with time,and then continued to rise with age(P<0.01).Conclusion Aging could increase the expression of OPG and the ratio of OPG/RANKL and decrease the expression of RANKL in HPDLCs under continuous static pressure in vitro.

关 键 词：增龄 正畸 人牙周膜细胞 持续静压力 骨保护素 核因子-ΚB受体活化因子配体 

分 类 号：R783.5[医药卫生—口腔医学]