FBXO22在急性髓系白血病中的表达分析  

Expression analysis of FBXO22 in acute myeloid leukemia

作　　者：吴冬会 杨艳丽[2] 周黎黎[2] 马丽[1] 汪晶 程阳 徐旺旺 李玉云[1] WU Dong-hui;YANG Yan-li;ZHOU Li-li;MA Li;WANG Jing;CHENG Yang;XU Wang-wang;LI Yu-yun(Key Laboratory of Cancer Research and Clinical Laboratory Diagnosis,School of Laboratory Medicine,Bengbu Medical College,Bengbu Anhui 233030;Department of Hematology,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,China)

机构地区：[1]蚌埠医学院肿瘤基础研究与临床检验诊断重点实验室,临床检验诊断学实验室中心,安徽蚌埠233030 [2]蚌埠医学院第一附属医院血液科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2022年第11期1573-1575,1579,共4页Journal of Bengbu Medical College

基　　金：安徽省高校自然科学研究重大项目(KJ2021ZD0080)。

摘　　要：目的:检测急性髓系白血病(AML)病人骨髓细胞FBXO22基因表达水平,分析其与病人临床指征的相关性。方法:收集AML病人92例,其中初发组41例,完全缓解(CR)组28例,部分缓解(PR)组23例,另选取肾性贫血、缺铁性贫血病人30例作为对照组。实时荧光定量PCR法测定并比较各组病人骨髓细胞中FBXO22 mRNA相对表达量。分析初发组病人FBXO22 mRNA相对表达量与病人性别、年龄、血常规参数、临床分型的相关性。结果:各组病人FBXO22 mRNA表达水平间差异有统计学意义(P<0.01),其中PR组和初发组均低于对照组和CR组(P<0.05),而对照组和CR组、PR组和初发组间差异均无统计学意义(P>0.05)。初发组病人中,不同血小板计数病人的FBXO22 mRNA表达水平差异有统计学意义(P<0.05)。相关分析显示,初发组病人血小板计数与FBXO22 mRNA相对表达水平呈明显正相关关系(r=0.492,P<0.01)。结论:AML病人骨髓细胞FBXO22表达降低,且与血小板计数呈正相关关系。Objective:To detect the expression level of FBXO22 gene in bone marrow cells of patients with acute myeloid leukemia(AML),and analyze its correlation with clinical indications.Methods:A total of 92 AML cases were collected,including 41 cases in the primary group,28 cases in the complete remission(CR)group,23 cases in the partial remission(PR)group,and 30 cases with renal anemia and iron deficiency anemia in the control group.The relative expression of FBXO22 mRNA in bone marrow cells was detected by real-time fluorescence quantitative PCR.The correlation between the expression of FBXO22 mRNA and the patient′s gender,age,blood routine parameters and clinical classification of patients were analyzed in the primary group.Results:There was a statistically significant difference in the expression level of FBXO22 mRNA among patients in each group(P<0.01).The levels of FBXO22 mRNA in the PR group and primary group were lower than those in the control group and CR group(P<0.05),but there was no statistically significant difference between the control group and CR group,PR group and primary group(P>0.05).There was significant difference in the expression level of FBXO22 mRNA among patients with different platelet counts in the primary group(P<0.05).Correlation analysis showed that there was a significant positive correlation between the platelet count and the relative expression level of FBXO22 mRNA in the primary group(r=0.492,P<0.01).Conclusions:The expression of FBXO22 in bone marrow of patients with AML is decreased,and positively associates with platelet count.

关 键 词：急性髓系白血病 FBXO22 血小板计数 

分 类 号：R733.7[医药卫生—肿瘤]