The chromosome-scale genome of Phoebe bournei reveals contrasting fates of terpene synthase (TPS)-a and TPS-b subfamilies  

作　　者：Xiao Han Junhong Zhang Shuang Han Sun Li Chong Guanliang Meng Minyan Song Yang Wang Shengcai Zhou Chengcheng Liu Luhuan Lou Xiongzhen Lou Longjun Cheng Erpei Lin Huahong Huang Qi Yang Zaikang Tong 

机构地区：[1]State Key Laboratory of Subtropical Silviculture,College of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou,Zhejiang 311300,China [2]Nextomics Biosciences,Wuhan,Hubei 430000,China [3]Experimental Forest Farm of Qingyuan County,Qingyuan,Zhejiang 323800,China

出　　处：《Plant Communications》2022年第6期26-42,共17页植物通讯（英文）

基　　金：supported by the Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding(grant 2021C02070-10);the National Natural Science Foundation of China(grants 32171828 and 32101545);the State Key Laboratory of Subtropical Silviculture(grant ZY20180204).

摘　　要：Terpenoids,including aromatic volatile monoterpenoids and sesquiterpenoids,function in defense against pathogens and herbivores.Phoebe trees are remarkable for their scented wood and decay resistance.Unlike other Lauraceae species investigated to date,Phoebe species predominantly accumulate sesquiterpenoids instead of monoterpenoids.Limited genomic data restrict the elucidation of terpenoid variation and functions.Here,we present a chromosome-scale genome assembly of a Lauraceae tree,Phoebe bournei,and identify 72 full-length terpene synthase(TPS)genes.Genome-level comparison shows pervasive lineage-specific duplication and contraction of TPS subfamilies,which have contributed to the extreme terpenoid variation within Lauraceae species.Although the TPS-a and TPS-b subfamilies were both expanded via tandem duplication in P.bournei,more TPS-a copies were retained and constitutively expressed,whereas more TPS-b copies were lost.The TPS-a genes on chromosome 8 functionally diverged to synthesize eight highly accumulated sesquiterpenes in P.bournei.The essential oil of P.bournei and its main component,b-caryophyllene,exhibited antifungal activities against the three most widespread canker pathogens of trees.The TPS-a and TPS-b subfamilies have experienced contrasting fates over the evolution of P.bournei.The abundant sesquiterpenoids produced by TPS-a proteins contribute to the excellent pathogen resistance of P.bournei trees.Overall,this study sheds light on the evolution and adaptation of terpenoids in Lauraceae and provides valuable resources for boosting plant immunity against pathogens in various trees and crops.

关 键 词：Phoebe bournei genome sequence terpene synthases SESQUITERPENOIDS positive selection pathogen resistance 

分 类 号：Q943.2[生物学—植物学]