五类马铃薯病毒实时荧光定量PCR检测方法的建立  

Establishment of Real-time Quantitative PCR Detection Method for Five Potato Viruses

作　　者：阳腾 刘怒安 刘聪聪 陈恩发 YANG Teng;LIU Nu’an;LIU Congcong;CHEN Enfa(Guizhou Institute of Biological Technology,Guiyang 550006,China)

机构地区：[1]贵州省生物技术研究所,贵阳550006

出　　处：《种子》2022年第10期135-138,共4页Seed

基　　金：黔农科院青年科技基金“马铃薯种薯不同代数退化与病毒的相关性研究”([2020]23号);省科研机构创新能力建设专项资金项目“贵州薯芋作物病害防控创新能力建设”(黔科合服企[2021]11号);“贵州省马铃薯现代农业产业技术体系建设”(GZCYTX 2022);黔农科院青年科技基金“薯芋类资源收集、保存与创制利用”([2022]23号)。

摘　　要：为提高马铃薯病毒检测效率和检测通量,采用酶联免疫吸附法(ELISA)和实时荧光定量PCR(qRT-PCR)检测技术对马铃薯苗中X病毒、Y病毒、S病毒、卷叶病毒及纺锤状块茎类病毒进行检测,以期构建五种马铃薯病毒的检测方法。结果表明,qRT-PCR法阳性检测率为6%,灵敏度比DAS-ELISA法高。qRT-PCR检测方法特异性强,重复性好,灵敏度高,可有效检测马铃薯的五种病毒。To improve the detection efficiency and throughput of potato virus,enzyme-linked immunosorbent assay(ELISA)and real-time PCR(qRT-PCR)were used in this study to detect X virus,Y virus,Svirus,leaf curl virus and spindle tuber virus in potato seedlings,in order to construct the detection method for five kinds of potato viruses.The results showed that the positive detection rate of qRT-PCR was 6%,and the sensitivity was higher than that of DAS-ELISA.The qRT-PCR method had strong specificity,good repeatability and high sensitivity,and could effectively detect five kinds of potato viruses.

关 键 词：马铃薯 病毒检测 实时荧光定量PCR 

分 类 号：S532[农业科学—作物学]