椰子ALDH基因家族的鉴定及生物信息学分析  

Identification and bioinformatics analysis of ALDH gene family in coconut

作　　者：武映宏 黄东益 王永[2] 张大鹏[2] 刘蕊[2] 孙熹微 肖勇[2] WU Yinghong;HUANG Dongyi;WANG Yong;ZHANG Dapeng;LIU Rui;SUN Xiwei;XIAO Yong(College of Tropical Crops,Hainan University,Haikou,Hainan 570228,China;Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences/Hainan Key Biological Laboratory of Tropical Oil Crops,Wenchang,Hainan 571339,China)

机构地区：[1]海南大学热带作物学院,海口570228 [2]中国热带农业科学院椰子研究所/海南省热带油料作物生物学重点实验室,海南文昌571339

出　　处：《热带生物学报》2022年第6期541-549,共9页Journal of Tropical Biology

基　　金：海南省重大科技计划项目(zdkj201902)。

摘　　要：为了鉴定椰子的ALDH基因家族,分析其基因结构、保守结构域、系统发育树以及其表达模式,通过下载OsALDH基因家族的蛋白质序列,并与椰子的蛋白质的数据库进行比对,鉴定出12个CnALDH基因,分属于9个亚族。结果表明,ALDH编码的氨基酸等电点为5.35~8.68,多数分布在叶绿体中。表达谱分析结果显示,除CnALDH12_A1和CnALDH22_A1外,其他的CnALDH基因家族成员均在叶片中有高水平表达。而CnALDH10_A8在不同组织以及不同时期展现组成性表达,推测该基因参与椰子的一些基础性功能,是不可或缺的基因。Aldehyde dehydrogenase(ALDH),which dehydrogenates aldehydes into carboxylic acids,plays an important role in plant stress,development and fragrance synthesis.The protein sequences of OsALDH gene were downloaded and compared with the protein database of coconut,and 12 CnALDH genes were identified,which belonged to 9 subfamilies.The results showed that the isoelectric points of amino acids encoded by ALDH ranged from 5.35 to 8.68,and most of them were distributed in chloroplasts.Expression profile analysis showed that all ALDH gene family members were highly expressed in coconut young leaves except CnALDH12_A1 and CnALDH22_A1.CnALDH10_A8 showed constitutive expression in different tissues and at different periods,suggesting that CnALDH10_A8 is an indispensable gene involved in some basic functions in coconut.

关 键 词：椰子 ALDH基因家族 生物信息学分析 

分 类 号：S667.4[农业科学—果树学] Q78[农业科学—园艺学]