Quality Standard of Yi Herb Medicine Millettia dielsiana  

作　　者：Maliniu SHA Wenbing LI Dongmei SHA Meiyi TONG Jing WANG Xinjia YAN Yuan LIU 

机构地区：[1]College of Pharmacy,Southwest Minzu University,Chengdu 610041,China [2]Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory,Chengdu 610225,China [3]Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China,Chengdu 610225,China

出　　处：《Medicinal Plant》2022年第5期26-30,共5页药用植物：英文版

基　　金：Supported by National Key Research and Development Program of China(2018YFC1706101);Science and Technology Program of Sichuan Province(Key Research and Development Project)(2021YFS0043);Standard Improvement of Traditional Chinese Medicine(Ethnic Medicine)of Sichuan Provincial Drug Administration(510201202102305);2022 Leading Talents Support Program of National Ethnic Affairs Commission;University-level Innovation Team Special Project of Basic Research Funds for Central Universities and Colleges(ZYN2022067)。

摘　　要：[Objectives]The paper was to establish the quality standard of Yi herb medicine Millettia dielsiana.[Methods]Yi herb medicine M.dielsiana was qualitatively identified by microscopic identification and thin layer identification.The content of total isoflavones was determined by UV-Vis spectrophotometry,and the contents of moisture,total ash,acid-insoluble ash and extract were checked according to the method introduced in Chinese Pharmacopoeia(2020 edition).[Results]The microscopic identification features,including stone cells,fiber bundles,calcium oxalate square crystals,starch granules,pigment blocks,ducts,etc.,were distinct.A TLC method for identification of M.dielsiana was established.The contents of water,total ash,acid-insoluble ash and extract should be no more than 13%,no more than 7%,no more than 0.5%and no less than 6%in 10 batches of samples,respectively.The total isoflavones of M.dielsiana based on genistein(C_(15)H_(10)O_(5)) should be no less than 0.25%.The linear relationship of genistein was good in the range of 0.002-0.007 mg/mL(R^(2)=0.9996);the average recovery was 97.01%and the RSD was 2.62%.[Conclusions]The method is simple,accurate and well-reproducible,and can be used for the quality control of Yi herb medicine M.dielsiana.

关 键 词：Yi herb medicine Millettia dielsiana GENISTEIN TLC UV-Vis spectrophotometry 

分 类 号：R29[医药卫生—民族医学]