大黄鱼变形假单胞菌的遗传学和血清学聚类特征初步分析  

A Preliminary Study on Genetic and Serological Clustering of Pathogenic Pseudomonas plecoglossicida in Larimichthys crocea

作　　者：许斌福[1] 陈秀锦[2] 池洪树[1] 徐梦婷 陈佳 林能锋[1] 龚晖[1] XU Binfu;CHEN Xiujin;CHI Hongshu;XU Mengting;CHEN Jia;LIN Nengfeng;GONG Hui(Institute of Biotechnology,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China;Fujian Center for Disease Control and Prevention,Fuzhou,Fujian 350002,China;State Key Laboratory of Large Yellow Croaker Breeding,Ningde,Fujian 352103,China)

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003 [2]福建省疾病预防控制中心,福建福州350002 [3]大黄鱼育种国家重点实验室,福建宁德352103

出　　处：《福建农业学报》2022年第9期1109-1116,共8页Fujian Journal of Agricultural Sciences

基　　金：福建省科技重大专项(2021NZ033025);福建省科技计划公益类专项(2019R1027-8、2020R1022009);福建省科特派后补助项目(2022S2095)。

摘　　要：【目的】内脏白点病是冬春季大黄鱼(Larimichthys crocea)最主要的细菌性疾病,变形假单胞菌(Pseudomonas plecoglossicida)是内脏白点病的主要病原。分析大黄鱼来源的变形假单胞菌病原聚类特征,为大黄鱼变形假单胞菌流行规律研究和科学防控提供参考。【方法】克隆7株不同时空来源的大黄鱼变形假单胞菌16S rDNA、gyrB,测序并构建进化树;制备上述菌株的O-特异链血清(O抗原血清)与鞭毛蛋白血清(H抗原血清),进行菌体凝集试验。【结果】16S rDNA和gyrB序列分析表明,所有的变形假单胞菌聚为一簇;O抗原和H抗原凝集结果表明,7株变形假单胞菌以及变形假单胞菌标准株均会发生凝集反应,凝集效价也无明显区别,与荧光假单胞菌(Pseudomonas fluoresenes)、溶藻弧菌(Vibrio alginolyticus)和哈维氏弧菌(Vibrio harveyi)不发生凝集反应。【结论】本研究所用的7株不同时空来源的变形假单胞菌亲缘关系接近,可能具有共同的起源。【Objective】Clustering of strains of Pseudomonas plecoglossicida,a major pathogen that causes the serious bacterial visceral white spot disease on Larimichthys crocea in winter and spring,was studied for disease control and epidemic prevention.【Method】The 16S rDNA and gyrB of 7 suspected P.plecoglossicida strains collected from different temporal and spatial sources were cloned and sequenced with evolutionary trees constructed.O and H antigen serums of these strains were prepared for agglutination tests.【Result】The 7 pathogens could be clustered into a same branch based on their sequences.Their O and H antigen sera agglutinated with those of the standard P.plecoglossicida reference without significant differences on titers,but not with that of P.fluoresenes,Vibrio alginolyticus,or V.harveyi.【Conclusion】It appeared that the 7 collected strains of P.plecoglossicida were genetically closely related and might have a common origin.

关 键 词：大黄鱼 变形假单胞菌 16S rDNA GYRB 血清菌体凝集 聚类分析 

分 类 号：S941[农业科学—水产养殖] Q933[农业科学—水产科学]