电针对雌性生长期营养性肥胖大鼠性发育水平及卵巢雌激素受体β表达的影响  

Effects of electroacupuncture on sexual development and ovarian estrogen receptorβexpression in female adolescent obese rats

作　　者：刘莉莉[1] 阎国强[2] 梁震[2] 赵丹慧[2] 丁宜[3] 段芳芳 万乃君[5] LIU Li-li;YAN Guo-qiang;LIANG Zhen;ZHAO Dan-hui;DING Yi;DUAN Fang-fang;WAN Nai-jun(Department of Acupuncture,Beijing Jishuitan Hospital,Beijing,100035,China;Beijing Institute of Traumatology and Orthopedics,Beijing Jishuitan Hospital,Beijing,100035,China;Department of Pathology,Beijing Jishuitan Hospital,Beijing,100035,China;Clinical Epidemiology Research Centre,Beijing Jishuitan Hospital,Beijing,100035,China;Department of Pediatrics,Beijing Jishuitan Hospital,Beijing,100035,China)

机构地区：[1]北京积水潭医院针灸科,北京100035 [2]北京积水潭医院北京市创伤骨科研究所,北京100035 [3]北京积水潭医院病理科,北京100035 [4]北京积水潭医院临床流行病学研究室,北京100035 [5]北京积水潭医院儿科,北京100035

出　　处：《针刺研究》2022年第10期896-901,共6页Acupuncture Research

基　　金：北京积水潭医院“学科新星”计划专项(No.XKXX201817)。

摘　　要：目的:观察电针对高脂饮食诱导的雌性生长期肥胖大鼠性发育水平和卵巢雌激素受体β(ER-β)表达的影响,探讨电针治疗青少年肥胖的部分作用机制。方法:21日龄雌性SD大鼠随机分为对照组、模型组和针刺组,每组6只。采用高脂饲料喂养6周建立肥胖大鼠模型。针刺组给予双侧“三阴交”“丰隆”“足三里”电针治疗,连续波,频率2 Hz,强度0.5~1.2 mA,30 min/d,连续干预14 d。治疗前、后测量大鼠体质量和腹围;ELISA法检测大鼠血清卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E_(2))含量;HE染色法观察大鼠卵巢黄体及卵泡数量;荧光定量PCR法检测大鼠卵巢ER-βmRNA的表达水平;Western blot法检测大鼠卵巢组织ER-β蛋白的表达水平。结果:与对照组比较,模型组大鼠体质量、腹围明显升高(P<0.05),血清FSH、E_(2)含量和卵巢ER-βmRNA、蛋白的表达明显升高(P<0.05),卵巢组织空卵泡及黄体数量明显增加;与模型组比较,针刺组大鼠体质量、腹围明显降低(P<0.05),血清FSH、E_(2)含量及卵巢ER-βmRNA、蛋白的表达明显降低(P<0.05),卵巢组织空卵泡数目及黄体数量降低,可见不同发育阶段的卵泡。结论:电针可以改善高脂饮食诱导的雌性生长期肥胖大鼠的性激素水平,改善卵巢组织发育,调节卵巢ER-βmRNA及蛋白的表达水平,从而调节雌性生长期肥胖大鼠性发育水平。Objective To observe the effect of electroacupuncture on sexual development and ovarian estrogen receptorβ(ER-β)expression in female adolescent obese rats induced by high-fat diet,so as to explore its underlying mechanisms of improving adolescent obesity.Methods Female SD rats(age of 21 days)were randomly divided into control,model and acupuncture groups,with 6 rats in each group.The obese model was established by feeding high-fat diet for 6 weeks.Rats of the acupuncture group received electroacupuncture(2 Hz,0.5-1.2 mA)stimulation at bilateral“Sanyinjiao”(SP6),“Fenglong”(ST40)and“Zusanli”(ST36)for 30 min,once a day for 14 days.The body mass and abdominal circumference of rats were measured before and after treatment.The contents of serum follicle stimulating hormone(FSH),luteinizing hormone(LH)and estradiol(E)were detected by ELISA.The number of corpus luteum and follicle were observed by HE staining.The expression levels of ER-βmRNA and protein in ovary were detected by fluorescence quantitative PCR and Western blot,respectively.Results Compared with the control group,the body mass and abdominal circumference,the contents of serum FSH and E,and the expression levels of ER-βmRNA and protein in ovary were significantly increased(P<0.05)in the model group,while the number of mature follicles and corpus luteum increased significantly.Compared with the model group,the body mass and abdominal circumference,the contents of serum FSH and E,and the expression levels of ER-βmRNA and protein in ovary were significantly decreased(P<0.05)in the acupuncture group,while the number of mature follicles and corpus luteum decreased significantly.Conclusion Electroacupuncture can effectively improve the levels of sex hormone and the development of ovary,down-regulate the expression levels of ER-βmRNA and protein in ovary,so as to regulate the process of sexual development of female adolescent obese rats induced by high-fat diet.

关 键 词：电针 高脂饮食 雌性大鼠 性发育 雌激素受体Β 

分 类 号：R245.97[医药卫生—针灸推拿学]