大肠杆菌发酵生产L-苯丙氨酸新工艺研究  

Study on New Technology of Producing L-Phenylalanine by Fermentation of Escherichia coli

作　　者：陈志超 李澜潇 徐庆阳[1] CHEN Zhi-chao;LI Lan-xiao;XU Qing-yang(College of Bioengineering,Tianjin University of Science and Technology,Tianjin 300450,China)

机构地区：[1]天津科技大学生物工程学院,天津300450

出　　处：《中国调味品》2022年第12期84-89,共6页China Condiment

基　　金：天津市科技计划项目(21ZYQCSY00050)。

摘　　要：为提高大肠杆菌发酵生产L-苯丙氨酸的产量与糖酸转化率,降低副产物积累,实验对发酵培养基中维生素H的用量进行了优化,并在此基础上优化了发酵工艺。实验结果表明,最适维生素H浓度为0.5 g/L,最优发酵工艺采用底物培养基∶流加培养基为4∶6,流加培养基流加速度随罐内溶氧值变化变速流加至菌体量稳定期,维生素H全部加入流加培养基的全营养流加工艺,最终L-苯丙氨酸产量达到了92 g/L,糖酸转化率为27.7%,乙酸产量为0.54 g/L,谷氨酸产量为0.4 g/L。经过工艺的优化,提高了L-苯丙氨酸的产量与转化率,并大幅降低了副产物的产量,这对发酵法生产L-苯丙氨酸具有指导意义。In order to increase the yield of L-phenylalanine produced by fermentation of Escherichia coli and the sugar-acid conversion rate,and reduce the accumulation of by-products,the amount of vitamin H in fermentation medium is optimized.Based on this,the fermentation process is optimized.The experimental results show that the optimal concentration of vitamin H is 0.5 g/L,and the optimal fermentation process is total nutrient fed-batch process:the ratio of substrate medium to fed-batch medium is 4∶6,feeding acceleration of fed-batch medium varies with dissolved oxygen value in the tank and feeding with variable speed until the volume of bacteria is stable,and all vitamin H is added into the fed-batch medium.The yield of L-phenylalanine reaches 92 g/L,and the sugar-acid conversion rate is 27.7%.The yields of acetic acid and glutamic acid are 0.54 g/L and 0.4 g/L respectively.After the optimization of the process,the yield of L-phenylalanine and the sugar-acid conversion rate increase,and the yield of by-products greatly decreases,which is of guiding significance for the production of L-phenylalanine by fermentation.

关 键 词：大肠杆菌 L-苯丙氨酸 维生素H 全营养流加 

分 类 号：TS201.3[轻工技术与工程—食品科学]